Lymph node cells from mice infected with live Mycobacterium bovis BCG were fused with BW5147 cells after short-term culturing in vitro. Both mycobacterium- and self-reactive T-cell hybridomas were identified. Some T-cell hybridoma clones displayed dual reactivity to self and to self plus mycobacterial antigen but did so to a different degree, indicating that infection with mycobacteria stimulat...

Interleukin-18 (IL-18) and IL-12 play a critical role in the expression of cell-mediated immunity involved in host defense against intracellular pathogens. Both cytokines are produced by macrophages and act in synergy to induce gamma interferon (IFN-gamma) production by T, B, and natural killer cells. In the present study, we analyzed both cellular and humoral responses upon infection with IL-1...

A60 class bulkhead penetration piece is a fire-resistance apparatus installed on compartments to protect lives and prevent flame diffusion in case of fire accident ships offshore plants. In this study, approximate optimization with discrete variables was carried out for the design an (A60 BPP) using various meta-models multi-island genetic algorithms. Transient heat transfer analysis evaluate p...

In vitro characterization and comparison of responses to different classes of biologically active compounds can increase the utility of bioassays. In this study, the relative potencies (REPs) of mixtures of polychlorinated naphthalenes (PCNs), polychlorinated biphenyls (PCBs), and polycyclic aromatic hydrocarbons (PAHs), to induce in vitro ethoxyresorufin-O-deethylase (EROD) in PLHC-1 fish hepa...

Because CD1-restricted T cells lack CD4 but produce IFN-gamma in response to nonpeptide mycobacterial antigens, they could play a unique role in immunity to tuberculosis. We studied CD1-restricted T cells in the context of HIV infection by expanding CD4(-) T cell lines from 10 HIV-infected patients. Upon stimulation with Mycobacterium tuberculosis antigen or upon exposure to macrophages infecte...

The theory of magnetic symmetry in quasicrystals, described in a companion paper [Lifshitz & Even-Dar Mandel (2004). Acta Cryst. A60, 167-178], is used to enumerate all three-dimensional octagonal spin point groups and spin-space-group types and calculate the resulting selection rules for neutron diffraction experiments.

TLR9 activation is important for the maintenance of mycobacteria-elicited pulmonary granulomatous responses, hallmarks of protective immune responses following mycobacterial infection. However, the mechanism or mechanisms underlying this effect of TLR9 are not clear. Here, we show that Tlr9-deficient mice challenged with a Mycobacterium antigen display an altered Th17 cytokine profile, decrease...

CD4+ T cells are essential players for the control of mycobacterial infections. Several mycobacterial antigens have been identified for eliciting a relevant CD4+ T cell mediated-immune response, and numerous studies explored this issue in the context of Mycobacterium tuberculosis infection. Antigen 85 (Ag85), a highly conserved protein across Mycobacterium species, is secreted at the early phas...