The female genital tract has a complex microbial flora which varies with site, age of the patient, and hormonal background. Likewise, the quality and quantity of cervical and vaginal secretions vary with many factors including age, phase of the menstrual cycle, use of oral contraceptive, pregnancy, and presence of an intrauterine contraceptive device. Vaginal discharge may arise from the vagina...

This study aimed to investigate Mycoplasma species in the lungs of 500 geese with pneumonia from Kars region (Turkey) via cultural and molecular methods. The samples were cultured on Frey’s Broth Agar media. To identify a Growth Inhibition Test was used. identification continued species-specific PCR sequence analysis which provide amplification genes dnaX, pcrA, rpoB, 16S rRNA gene, respectivel...

Although treponemes are consistently identified in tissue from bovine digital dermatitis (DD) lesions, the definitive etiology of this debilitating polymicrobial disease is still unresolved. To study the microbiomes of 27 DD-infected and 10 healthy interdigital skin samples, we used a combination of different molecular methods. Deep sequencing of the 16S rRNA gene variable regions V1-V2 showed ...

The isolation of Mollicutes from food has not been reported. To isolate Mollicutes in the presence of high levels of unwanted bacteria, we first incubated fresh vegetables in liquid culture media containing lysozyme, ampicillin, and thallous acetate. Culture fluids were than separated from the vegetable samples, subjected to one freeze-thaw cycle, and passed through a filter of 0.4-micron poros...

Hemotrophic mycoplasmas (hemoplasmas) are a group of animal pathogens of the Mollicutes class. Recently, the genomes of 8 hemoplasmas have been completely sequenced. The aim of this study was to gain a better understanding of their genomic features and relationship to other Mycoplasma species. The genome structure and dynamics of hemoplasmas were analyzed by evaluating gene synteny, adaptive ev...

In October 2016, Seattle Children's Hospital notified the Washington State Department of Health (DOH) and CDC of a cluster of acute onset of limb weakness in children aged ≤14 years. All patients had distinctive spinal lesions largely restricted to gray matter detected by magnetic resonance imaging (MRI), consistent with acute flaccid myelitis (AFM). On November 3, DOH issued a health advisory ...

A common characteristic of Mycoplasma diseases is their chronicity, spp. infection have been frequently associated with upper respiratory infections, chronic lung disease , asthma, meningeal encephalitis, mastitis, arthritis, heart problems, sterility, bone problems oviduct dysfunctions and death in human different animal species. In former years, changeable expression membrane antigens has det...

BACKGROUND Bacterial vaginosis (BV) - a syndrome characterised by a shift in vaginal flora - appears to be particularly common in sub-Saharan Africa, but little is known of the pattern of vaginal flora associated with BV in Africa. We conducted a study aimed at determining the prevalence of BV and patterns of BV-associated vaginal micro-flora among women with vaginal discharge syndrome (VDS) in...