The central nervous system myelin sheath is a multilayered specialized membrane with compacted and non-compacted domains of defined protein composition. How oligodendrocytes regulate myelin membrane trafficking and establish membrane domains during myelination is largely unknown. Oligodendroglial cells respond to neuronal signals by adjusting the relative levels of endocytosis and exocytosis of...

Twenty-three rabbit microsatellites were extracted from the EMBL nucleotide database. Nine of these markers, together with nine earlier published microsatellite markers, were found to be polymorphic between the AX/JU and IIIVO/JU inbred strains. By using an F(2) intercross we could integrate five markers into the rabbit linkage map. One anonymous microsatellite marker could be assigned to chrom...

Oligodendrocytes generate large amounts of myelin by extension of their cell membranes. Though lipid is the major component of myelin, detailed lipid metabolism in the maintenance of myelin is not understood. We reported previously that miR-32 might be involved in myelin maintenance (Shin et al., 2009). Here we demonstrate a novel role for miR-32 in oligodendrocyte function and development thro...

Cover Illustration: Confocal image of a mature oligodendrocyte in vitro following the addition netrin-1, immuno-labelled for mitochondrial docking protein syntaphilin (red), outer membrane TOM20 (green), myelin basic (white), and Hoechst dye (blue). Syntaphilin is associated with mitochondria oligodendrocytes recruited to cell processes by netrin-1. (See Nakamura, DS, et al. https://doi.org/10....

The lipid composition was determined for axolemma-enriched fractions and myelin which were isolated via a preparation of purified myelinated axons. The myelin had a lipid composition which was compatible with that previously reported for myelin isolated by alternative procedures. The most dense axolemma-enriched fraction contained 25.3% cholesterol, 25.8% galactolipid (21.3% cerebrosides and 4....

Two inactive/latent protein phosphatases termed LP-1 (Mr 260,000) and LP-2 (Mr 350,000) were identified and purified from pig brain. Examination of molecular structures indicated that LP-1 has three subunits with molecular weights of 69,000, 55,000, and 34,000, respectively, whereas LP-2 contains only one subunit, with molecular weight of 49,000. When using phosphorylase a as a substrate, LP-1 ...

We analyzed the location and abundance of transcripts for the 4 CNS myelin protein genes, myelin basic protein (MBP), proteolipid protein (PLP), myelin-associated glycoprotein (MAG), and 2',3'-cyclic nucleotide phosphohydrolase (CNP), in the mouse cervical spinal cord from the time of rapid myelination until adulthood (8-45 d). In the white matter, maximal levels of transcripts were found for e...