The trml mutation of Saccharomyces cerevisiae is a single nuclear mutation that affects a specific base modification of both cytoplasmic and mitochondrial tRNA. Transfer RNA isolated from trml cells lacks the modified base N2,N2-dimethylguanosine, and extracts from these cells do not have detectable N2,NZdimethylguanosine-specific tRNA methyltransferase activity. As part of our efforts to deter...

Molecular dynamic simulation program has been developed which can be used to model the static and dynamic properties of molecular nitrogen (N2) under high pressure. The dynamics of the N2 molecules accounts precisely both translational and rotational degrees of freedom. The rotational motion of the N2 molecule is approximated assuming constant moment of inertia of the nitrogen molecule. The pro...

M(N2)n(+) (M = Y, La, Ce; n = 7-8) complexes have been studied by infrared photodissociation (IRPD) spectroscopy and density functional theory (DFT) calculations. The experimental results indicate that the N-N stretching vibrational frequencies are red-shifted from the gas-phase N2 value. The π back-donation is found to be a main contributor in these systems. IRPD spectra and DFT calculations r...

Decreased N2 fixation in soybean (Glycine max) L. Merr. during water deficits has been associated with increases in ureides and free amino acids in plant tissues, indicating a potential feedback inhibition by these compounds in response to drought. We evaluated concentrations of ureides and amino acids in leaf and nodule tissue and the concurrent change in N2 fixation in response to exogenous u...

Excessive anthropogenic nitrogen (N) and phosphorus (P) inputs have caused an alarming increase in harmful cyanobacterial blooms, threatening sustainability of lakes and reservoirs worldwide. Hypertrophic Lake Taihu, China's third largest freshwater lake, typifies this predicament, with toxic blooms of the non-N2 fixing cyanobacteria Microcystis spp. dominating from spring through fall. Previou...

12 Benthic nitrogen (N2) fixation and sulfate reduction (SR) were investigated in the Peruvian 13 oxygen minimum zone (OMZ). Sediment samples, were retrieved by a multiple corer at six 14 stations (70 1025 m water depth) along a depth transect at 12°S, covering anoxic and 15 hypoxic bottom water conditions. Benthic N2 fixation was detected at all sites using the 16 acetylene reduction assay, wi...

A nitrogenase-inspired biomimetic chalcogel system comprising double-cubane [Mo2Fe6S8(SPh)3] and single-cubane (Fe4S4) biomimetic clusters demonstrates photocatalytic N2 fixation and conversion to NH3 in ambient temperature and pressure conditions. Replacing the Fe4S4 clusters in this system with other inert ions such as Sb(3+), Sn(4+), Zn(2+) also gave chalcogels that were photocatalytically a...

Nitrogen fixation in waterlogged, soil-straw, and sandday-straw mixtures was measured with the C2H2reduction assay, the I5N-tracer technique, and the Kjeldahl method. The reduction of 6 to 15 moles C2H2 corresponded to the fixation of 1 mole N2. The theoretical ratio is 3 moles C2H2 to 1 mole N2. A ratio of 3 moles C2H2 reduced for each mole of N2 fixed was obtained when samples of sandxlay-str...