The role of centrosomes and centrioles during mitotic spindle assembly in vertebrates remains controversial. In cell-free extracts and experimentally derived acentrosomal cells, randomly oriented microtubules (MTs) self-organize around mitotic chromosomes and assemble anastral spindles. However, vertebrate somatic cells normally assemble a connected pair of polarized, astral MT arrays--termed a...

STUDY OBJECTIVE To compare the safety and efficacy of metered-dose inhaler (MDI) albuterol to nebulized (NEB) albuterol administration. DESIGN A randomized, triple-blinded, crossover study. SETTING A pediatric ICU in a tertiary care children's hospital. PATIENTS Eleven intubated infants with bronchiolitis. INTERVENTIONS Subjects received four puffs of MDI albuterol (360 microg) and 3 mL...

O conforto térmico, Humidex, é uma variável muito importante para avaliar o grau de estresse térmico em seres vivos. Por depender variáveis climáticas, Humidex tem variação espaço-tempo-intensidade condicionada a vários atenuadores do tempo e clima. Assim, objetivo deste trabalho verificar possíveis tendências análises espaço-temporais índice no Nordeste Brasil (NEB). Foram utilizados os dados ...

Proper centrosome separation is a prerequisite for positioning the bipolar spindle. Although studies demonstrate that microtubules (MTs) and their associated motors drive centrosome separation [1], the role of actin in centrosome separation remains less clear. Studies in tissue culture cells indicate that actin- and myosin-based cortical flow is primarily responsible for driving late centrosome...

Many meiotic systems in female animals include a lengthy arrest in G2 that separates the end of pachytene from nuclear envelope breakdown (NEB). However, the mechanisms by which a meiotic cell can arrest for long periods of time (decades in human females) have remained a mystery. The Drosophila Matrimony (Mtrm) protein is expressed from the end of pachytene until the completion of meiosis I. Lo...

The nudged elastic band method (NEB) can be used to find a minimum energy path between two given starting structures. This method has been available in the standard release of the Amber9 and Amber10 suite of programs. In this paper a novel implementation of this method will be discussed, in which the nudged elastic band method is applied to only a specific, user-defined subset of atoms in a par...

In this paper, we present our ab-initio study on energy configurations, minimum energy path (MEP), and migration energy for neutral indium diffusion in a uniaxial and biaxial tensile strained {100} silicon layer. Our abinitio calculation of the electronic structure allowed us to figure out transient atomistic configurations during the indium diffusion in strained silicon. We found that the lowe...

A new implementation of the Nudged Elastic Band (NEB) optimization method is presented. This approach uses a global procedure that yields the whole reaction path, and thus it provides an alternative to the sequential optimization of the transition state and consequent calculation of the minimum energy path. Furthermore the algorithm is very useful when one is not sure if a saddle point exists, ...

Compared to other analytical platforms, comprehensive two-dimensional gas chromatography coupled with mass spectrometry (GC×GC-MS) has much increased separation power for analysis of complex samples and thus is increasingly used in metabolomics for biomarker discovery. However, accurate peak detection remains a bottleneck for wide applications of GC×GC-MS. Therefore, the normal-exponential-Bern...