methods: we examined 170 formalin-fixed paraffin-embedded samples from esophageal squamous cell carcinoma patients. all subjects live in mazandaran province, a region with high incidence rate of esophageal cancer and have become known as the “asian esophageal cancer belt”. samples were tested for hpv-dna by my09/11 and gp5+/6+ general primers using nested pcr. results: of the 170 escc samples, ...

background : the protozoan parasites cryptosporidium spp. and giardia are known to occur widely in both raw and drinking waters. they are two of the causative agents of waterborne out-breaks of gastroenteritis throughout the world. in the present study, a pcr assay and fa were developed for detection of cryptosporidium oocysts and giardia cyst in environmental samples. methods : we have detecte...

conclusions our study demonstrated that the b1 gene, more so than the re gene, showed positive samples and can be used to detect toxoplasmosis, although the b1 gene, in comparison to the re gene, did not show any superiority of molecular diagnosing capability. results also showed that toxoplasma molecular detection methods can be used instead of routine serological detection methods in a clinic...

A traditional nested reverse transcription-polymerase chain reaction (RT-PCR) assay specific for eastern equine encephalomyelitis (EEE) virus was designed to multiplex with a previously described West Nile (WN) virus nested RT-PCR assay. Differentiation of EEE and WN was based on base pair size of the amplified product. One hundred fifty-seven mammalian and avian brain tissues were tested by EE...

background and purpose: microsporidiosis is one of the emerging and opportunistic infections, which causing various clinical symptoms in humans. the prevalence of this infection varies, depending on the infected organ, diagnostic methods, and geographical conditions. in the present study, we aimed to investigate microsporidial keratitis in patients referring to farabi eye hospital tehran, iran ...

background: the diagnosis of ocular toxoplasmosis is mainly based on clinical features. however, ocular fluid testing by pcr may be very helpful for approval or rejection of this etiology. in this study, we utilized a nested-pcr technique, targeting the b1 partial sequence to analyze the aqueous and vitreous samples for evaluating the presence of the toxoplasma dna. methods: fifty aqueous or vi...

background: cryptosporidiosis is a common coccidian parasite infection in patients with diarrhea that has worldwide distribution especially in developed countries. therefore,the aim of this study was to determine the occurrence of cryptosporidium infection in patients with gastroenteritis admitted to hospitals of mazandaran university of medical sciences by parasitological and molecular methods...