clostridium septicum a gram positive anaerobic bacterium produces several toxins including alpha, beta, gamma and delta. c. septicum alpha toxin is lethal and is responsible for a serious disease known as gas gangrene. the aim of the present study was to molecular cloning and sequencing of c. septicum vaccine strain alpha toxin gene. genomic dna was extracted using standard phenol and chlorofor...

abstract the ability to monitor particular microorganism in an environment is a difficult task. many genes permit the differentiation of strains by conferring production of unique phenotypes such as bioluminescence [marine bacterial luciferase (luxab)]. therefore, lux-ab gene was cloned into two endemic plant pathogens pseudomonas syringae and ralostonia solanacearum by electrotransformation. l...

methyl tert-butyl ether (mtbe), a gasoline octane enhancer, was introduced as a substitute for lead tetraethyl over 30 years ago. widespread use of mtbe in gasoline, has introduced mtbe into the environment compartments, mostly into the under ground and surface water and water as a second most frequently detected contaminant. in this study, we have isolated pure cultures from bacterial consorti...

background: toxoplasma gondii , an apicomplexan parasite, is capable of infecting a broad range of intermediate warm-blooded hosts including humans. the parasite seems to be capable of altering the natural behavior of the host to favor its trans-mission in the environment. the aim of this study was to evaluate the course, alter-ations in behavior along with normal kinetics of the abnormally ind...

Tensile stress-strain curve is of high importance in mechanics of materials particularly in numerical simulations of material deformations. The curve is usually obtained by experiment but is limited by necking phenomenon. Engineering stress-strain curve is converted to true stress-strain curve through simple formulas. The conversion, however, is correct up the point of necking. From this point ...

  Genetic typing methods of T. gondii strains have been extensively perfected in recent years. From a technical point of view, many tools usable for genetic studied on single-copy loci have been used: RFLP, PCR-RFLP, sequencing, RAPD-PCR and isoenzyme analysis. We described the cloning and sequence analysis of the gene which encodes the major surface antigen (SAG1 or P30) of T. gondii. SAG1 is ...

The purpose of this investigation is to examine the viability of using the sheet metal for forming applications. Forming limit diagram (FLD) composed of negative and positive minor strain with respect to major strain which occurs at directional zero strain with the critical thickness of sheet metal. The negative minor strain region of FLD is predicted by localized necking. However there is no d...