RNA polymerase II synthesizes a diverse set of transcripts including both protein-coding and non-coding RNAs. One major difference between these two classes of transcripts is the mechanism of termination. Messenger RNA transcripts terminate downstream of the coding region in a process that is coupled to cleavage and polyadenylation reactions. Non-coding transcripts like Saccharomyces cerevisiae...

Why do we need Rfam? Rfam was established to make it easier to identify homologues of known non-coding RNA. We also provide comprehensive text and functional annotation [7] for these sequences. Computational analysis of non-coding RNAs is time consuming when compared to doing similar searches on protein. We provide access to RNA search tools and results which require a lot of computational time...

Several examples have come to light in which mutations in non-protein-coding regions give rise to a deleterious gain-of-function by non-coding RNA. Expression of the toxic RNA is associated with formation of nuclear inclusions and late-onset degenerative changes in brain, heart or skeletal muscle. In the best studied example, myotonic dystrophy, it appears that the main pathogenic effect of the...

Now, it is clear that protein is just one of the most functional products produced by the eukaryotic genome. Indeed, a major part of the human genome is transcribed to non-coding sequences than to the coding sequence of the protein. In this study, we selected three long non-coding RNAs namely AK082072, AK043754 and AK082467 which show brain expression and local region conservation among vertebr...

The assumption that RNA can be readily classified into either protein-coding or non-protein-coding categories has pervaded biology for close to 50 years. Until recently, discrimination between these two categories was relatively straightforward: most transcripts were clearly identifiable as protein-coding messenger RNAs (mRNAs), and readily distinguished from the small number of well-characteri...

objective:glioblastoma is an invasive tumor of the central nervous system. epigenetic therapy of cancer is potentially very useful in reversing some of cancer defects due to reversibility of epigenetic alterations. meg3 is a tumor suppressor long non-coding rna (lncrna) that expresses in the majority of normal tissues. methylation of the meg3 promoter region elicits a decrease in its expression...