Caged (that is, masked) fluorescent dyes are maintained in their nonfluorescent state by the incorporation of a photochemical labile group. The photosensitive masking group or “molecular cage” can be cleaved-off by irradiation with nearUV light, thereby rendering the dye fluorescent. Caged fluorescent dyes are of enormous interest for biological imaging because they may be used, for example, fo...

PURPOSE To evaluate systematically the staining characteristics and safety of potential new dyes for intraocular surgery. METHODS Six dyes were included in the investigation: light green SF (LGSF) yellowish, E68, bromophenol blue (BPB), Chicago blue (CB), rhodamine 6G, rhodulinblau-basic 3 (RDB-B3). All dyes were dissolved and diluted in a balanced saline saline solution. The light-absorbing ...

A novel layer-by-layer sequential adsorption technique based on electrostatic interaction is a powerful way to fabricate multilayer organic thin films. The advantages of this technique include control over the number of layers, the architecture of the multilayers, and their ease of fabrication. By controlling the conditions of the polyion dipping solutions, such as salt concentration, solution ...

The straightforward synthesis of two new classes of core-extended perylene chromophores, dibenzocoronene tetracarboxdiimide and indenoperylene dicarboxmonoimide, proves that directional enlargement of the aromatic pi-system leads to tailored bathochromic and hypsochromic shifts.

Flavin-dependent oxidoreductases are increasingly recognized as important biocatalysts for various industrial applications. In order to identify novel activities and to improve these enzymes in engineering approaches, suitable screening methods are necessary. We developed novel microtiter-plate-based assays for flavin-dependent oxidases and dehydrogenases using redox dyes as electron acceptors ...

The detail understanding of physiological/biochemical characteristics of individual laccase isoenzymes in fungi is necessary for fundamental and application purposes, but our knowledge is still limited for most of fungi due to difficult to express laccases heterologously. In this study, two novel laccase genes, named lac3 and lac4, encoding proteins of 547 and 532-amino acids preceded by 28 and...

Fluorescent markers emitting in the red are extremely valuable in biological microscopy since they minimize cellular autofluorescence and increase flexibility in multicolor experiments. Novel rhodamine dyes excitable with 630 nm laser light and emitting at around 660 nm have been developed. The new rhodamines are very photostable and have high fluorescence quantum yields of up to 80 %, long exc...

This work unveils the formation of novel aqueous biphasic systems (ABS) formed by perfluoroalkylsulfonate-based ionic liquids (ILs) and a large number of carbohydrates (monosaccharides, disaccharides and polyols) aiming at establishing more benign alternatives to the salts commonly used. The respective ternary phase diagrams were determined at 298 K. The aptitude of the carbohydrates to induce ...

It is a great challenge to trapping novel Bi2WO6/MIL-53(Al) (BWO/MIL) nanocomposites with excellence catalytic activity by the trial-and-error method in vast untapped synthetic space. The degradation rate of dyes Rhodamine...

The facile synthesis of the new class of core-extended rylene chromophores, dibenzopentarylenebis(dicarboximide)s, having an intense absorption at 1020-1040 nm, is presented.