The stability of host nucleic acids in L cells infected with Chlamydia psittaci (strain meningopneumonitis) was studied. The L cells were prelabeled with either (32)P-orthophosphate, (3)H-uridine, or (3)H-thymidine. After infection, the redistribution of each label among the different fractions of host and parasite was quantitatively determined and compared. There were no signs of accelerated d...

background: we examined a molecular method with a single-pcr for amplification of a part of cp5 gene enabling us to differen­tiate the pathogenic species, entamoeba histolytica , from the non-pathogenic species, e. dispar . methods: we developed a single pcr method for this purpose.  after investigation of genbank, primer pairs were de­signed from highly conserved regions of cysteine proteinase...

With the bone marrow of anemic rats, which had received the repeated injections of phenylhydrazine once a day for three to four days, the effects of aminopterin and bromouracil on the nucleic acid metabolism of erythroblasts were observed in vivo experiment. The injection of aminopterin suppreed DNA synthesis with the lowered labeling index as observed by the incorporation of Hthymidine into DN...

utilization patterns of hamsters and rats bearing human neoplasms. These studies showed that, in sofar as the synthesis of the purine moiety of their nucleic acids is concerned, these tumors possess distinctive characteristics as compared with the host tissues. In the synthesis of the nucleic acids, the neoplastic tissue used guanine and hypoxan thine inefficiently as compared with the host, bu...

The synthesis of host macromolecules was shut off very slowly and incompletely by bacteriophage SPO2c(1). No change in the rate of incorporation of radioactive precursors into protein and ribonucleic acid (RNA) could be detected after infection, and the rate of incorporation of thymidine was increased only slightly. The relative proportions of phage and host species of nucleic acids at various ...

Nucleotides are low molecular weight intracellular compounds that play major roles in physiological and biological functions. They act as precursors for nucleic acid synthesis and are also fundamental for intermediary metabolism. Nucleotides and nucleic acids turn over rapidly, especially in growing tissues or those undergoing constant cell renewal. Tissues that grow have a net formation of new...

The effect of aqueous garlic extract on the macromolecular synthesis of Candida albicans was studied. Protein and nucleic acid syntheses were inhibited to the same extent as growth, but lipid synthesis was completely arrested. Blockage of lipid synthesis is likely an important component of the anticandidal activity of garlic.

The recognition of the control mechanisms through which the cell maintains the specific properties of its high molecular components is condemned to ,being posthumous; it is necessary to kill or poison the cell before its way of life can be revealed. It is, therefore, not surprising that many of the pathways through which the cell accomplishes its integration have remained largely speculative. A...