Titers of wild-type and recombinant adeno-associated viruses are routinely determined by DNA slot blot analysis. The binding of viral DNA to nylon membranes was found to be inhibited by magnesium ions, which are critical components of the DNase I digestion carried out prior to slot blot analysis. Mg2+ions also interfered with the adsorption of plasmid DNA to nylon and nitrocellulose membranes. ...

Immunocompetent cells were isolated from spleen of B. himalayanus and studied surface morphology of the three different cell types--(i) plastic adherent; (ii) nylon wool adherent; and (iii) nylon wool non-adherent cells. As revealed by scanning electron microscopy, they resembled the macrophages, B and T cells, respectively. Presence of such cell types indicated that Bufo himalayanus possessed ...

Some exploratory work on creep and inverse creep in nylon 6 monofilament, polyester multifilament yarn and wool and cotton spun yarns has been presented. It is observed that the extent of creep and inverse creep differs from material to material and depends on the initial stress. Cotton shows minimum inverse creep while nylon shows maximum inverse creep. Wool and polyester have intermediate inv...

Nylon-3 polymers (poly-β-peptides) have been investigated as synthetic mimics of host-defense peptides in recent years. These polymers are attractive because they are much easier to synthesize than are the peptides themselves, and the polymers resist proteolysis. Here we describe in vitro analysis of selected nylon-3 copolymers against Clostridium difficile, an important nosocomial pathogen tha...

Fungal infections are a major challenge to human health that is heightened by pathogen resistance to current therapeutic agents. Previously, we were inspired by host-defense peptides to develop nylon-3 polymers (poly-β-peptides) that are toxic toward the fungal pathogen Candida albicans but exert little effect on mammalian cells. Based on subsequent analysis of structure-activity relationships ...

Synthetic versions of human derived kairomones can be used as baits when trapping host seeking mosquitoes. The effectiveness of these lures depends not only on their attractiveness to the mosquitoes but also on the medium from which they are dispensed. We report on the development and evaluation of nylon strips as a method of dispensing odorants attractive to the malaria vector, Anopheles gambi...

Due to the recent development of wearable electronic textiles and smart textiles, the conductive fabrics and electrically heated garments have been extensively studied and applied in many fields. In this work an electric heating fabric was fabricated by sewing silver coated nylon yarn over the polyester fabric. In order to minimize the power consumption, instead of heating whole garment surface...

Dot and slot blotting are simple techniques for immobilizing bulk unfractionated DNA on a nitrocellulose or nylon membrane. Hybridization analysis can then be carried out to determine the relative abundance of target sequences in the blotted DNA preparations. Dot and slot blots differ only in the geometry of the blot, a series of spots giving a hybridization pattern that is amenable to analysis...

The electrostatic charge on nylon, polypropylene (PP), and polytetrafluoroethylene (PTFE) after rubbing against stainless steel and against each other was examined using a newly developed rubbing device with automated features. It is observed that the charge generated on PTFE and PP increases as the contact force increases, while the charge generated on nylon is not affected by the contact forc...