BACKGROUND Amelogenin is required for normal enamel formation and is the most abundant protein in developing enamel. METHODS Amelx+/+, Amelx+/- , and Amelx-/- molars and incisors from C57BL/6 mice were characterized using RT-PCR, Western blotting, dissecting and light microscopy, immunohistochemistry (IHC), transmission electron microscopy (TEM), scanning electron microscopy (SEM), backscatte...

Reproducible time-of-flight cluster static secondary ion mass spectra (ToF-SSIMS) were obtained for various standard calcium phosphate (CP) powders, which allowed for phase identification. X-ray diffraction was not able to detect signals from microscopic amounts of CP (∼15 mmol m(-)(2)). The phases studied were α-tricalcium phosphate [α-Ca(3)(PO(4))(2)], β-tricalcium phosphate [β-Ca(3)(PO(4))(2...

Calcium phosphate is attracting attention as a bone repair material and controlled-release carrier of various drugs such disease therapeutic agents anticancer agents. Compared with some bioabsorbable polymers, calcium phosphates have the advantage preventing pH decrease in surrounding body fluid. However, there are few studies comparing effect supporting substances different physicochemical pro...

The present author and coworkers have engaged in the research developments of inorganic phosphate materials with high performance, utilizing characteristic properties phosphates forms crystals glasses. review described our previous regarding (i) preparation fibrous octacalcium (Ca8H2(PO4)6∙5H2O) hydroxyapatite (Ca10(PO4)6(OH)2; HAp) particles due to anisotropic crystal growth, (ii) superplastic...

This article presents the results of a study radiation-induced defects in various synthetic calcium phosphate (CP) powder materials (hydroxyapatite—HA and octacalcium phosphate—OCP) by electron paramagnetic resonance (EPR) spectroscopy at X, Q, W-bands (9, 34, 95 GHz for microwave frequencies, respectively). Currently, CP are widely used orthopedics dentistry owing to their high biocompatibilit...

The hydroxyapatite (HAP) with variable chemical substitutions has been considered as the major component in the mineralized part of bones. Various metastable crystalline phases have been suggested as transitory precursors of HAP in bone, but there are no consensuses as to the nature of these phases and their temporal evolution. In the present study, we cultured rat calvarial osteoblasts with as...