Olfactory coding at the level of the olfactory bulb is thought to depend upon an ensemble response of mitral cells receiving input from chemotopically-organized projections of olfactory sensory neurons and regulated by lateral inhibitory circuits. Immunocytochemical methods are described to metabolically classify neurons in the developing zebrafish olfactory system based on the relative concent...

Disturbances of olfaction are a common occurrence in many neurological and neurosurgical patients and their correct diagnosis might be helpful in management and enhancement of quality of life. However, olfaction is seldom checked in most neurosurgical units and the "smell bottles" are often either absent or out of date. This chapter reviews systematically recent advances in our understanding of...

In mammals, the detection of pheromones is mediated by the vomeronasal system. We have employed gene targeting to visualize the pattern of projections of axons from vomeronasal sensory neurons in the accessory olfactory bulb. Neurons expressing a specific receptor project to multiple glomeruli that reside within spatially restricted domains. The formation of this sensory map in the accessory ol...

Olfactory receptor usage is highly non-uniform, with some receptor types being orders of magnitude more abundant than others. Intriguingly, in mammals the receptors are replaced regularly and the receptor distribution changes in response to olfactory experience. We propose a theoretical model that explains these experimental observations. Our model suggests that the receptor distribution is tun...

Transduction mechanisms were investigated in human olfactory neurons by determining characteristics of odorant-induced changes in intracellular calcium concentration ([Ca2+]i). Olfactory neurons were freshly isolated from nasal biopsies, allowed to attach to coverslips, and loaded with the calcium-sensitive indicator fura-2. Changes in [Ca2+]i were studied in response to exposure to individual ...

The olfactory system permits animals to recognize and discriminate a vast number of different odorous molecules. This sensitivity is achieved by the selective expression of a single odorant receptor, specific to a small subset of the universe of odors, in each olfactory sensory neuron [1,2]. The topography of synaptic connections at the first relay in the olfactory system is organized according...

Odorant/receptor binding and initial olfactory information processing occurs in olfactory receptor neurons (ORNs) within the olfactory epithelium. Subsequent information coding involves high-frequency spike synchronization of paired mitral/tufted cell dendrites within olfactory bulb (OB) glomeruli via positive feedback between glutamate receptors and closely-associated gap junctions. With mRNA ...

Stimulating arbitrary collections of as few as 300 neurons in the primary olfactory cortex of mice suffices for associative learning independent of any odor stimulation. Thus, programmed spatial relationships may not exist in piriform cortex, making flexible random associations the rule.