(ADEM) was made based on elevated oligoclonal IgG bands, myelin basic protein level, and 80% monocytes in the CSF with absence of serum antibodies against commonly causal viruses. Magnetic resonance imaging (MRI) indicated extensive damage to the brainstem and cerebellum, A 44-year-old man was hospitalized for meningitis, with increased cell count and protein level in the cerebrospinal fluid (C...

A two-step procedure is described in which analytical disc gel electrophoresis in polyacrylamide is used to demonstrate oligoclonal bands of immunoglobulin G in cerebrospinal fluid. The principal advantage of the procedure is that unconcentrated samples of cerebrospinal fluid as small as 200 fiL can be assayed. The results obtained in samples from 100 patients with various neurologic diseases s...

Agarose and/or cellulose acetate electrophoresis was performed on the CSF of one thousand patients. In patients with clinically definite multiple sclerosis (MS) (N = 267) 92.8% had oligoclonal banding (O.B.). In patients with possible MS (N = 283) O.B. was present in 31.1%. In patients with other neurological diseases (N = 450) O.B. was present in 8% (N = 36). Nineteen non-MS patients with posi...

We describe use of a microconcentrator membrane with a 30 000-Da cutoff for treatment of cerebrospinal fluid (CSF) specimens before detection of oligoclonal bands by electrophoresis on agarose. After centrifugation at 2000 X g for 25 min, 0.5-, 1.0-, and 2.0-mL aliquots of CSF were concentrated 15-, 25-, and 40-fold. Analytical recovery of immunoglobulins G and A from the microconcentrators was...

The detection of oligoclonal bands of IgG in cerebrospinal fluid by isoelectric focusing is a useful aid in the diagnosis of multiple sclerosis. At present, this test is performed on polyacrylamide gels which are technically very difficult to prepare and are not suitable for the routine laboratory. A technique is described for isoelectric focusing on thin layer agarose gels which permits oligoc...

A selection of 58 specimens with a monoclonal component identified by immunoelectrophoresis and/or immunofixation was analyzed with the immunosubtraction procedure on the Paragon 2000 capillary electrophoresis system. The capillary system detected 93% of the paraproteins and, using immunosubtraction, correctly identified 91% of the paraproteins. Paraproteins that were detected by immunofixation...

Chronic intrathecal immunoglobulin (Ig) production is a hallmark of multiple sclerosis characterized by the presence of oligoclonal IgGs and, in addition, polyspecific recognition of different pathogens such as measles, rubella and herpes zoster virus. While the antigen specificity of the oligoclonal IgGs in multiple sclerosis is largely unknown, the oligoclonal IgGs arising during CNS infectio...