The principle of isotachophoresis has been used to develop a simple, specific and sensitive analytical method for the determination of anions in untreated urine. Isotachophoretic separation of anions in the presence of complex-forming counter ions (e.g. Cd2+) enables the quantitative separation and determination of chloride, sulphate and orthophosphate in the same sample and the less accurate m...

Microsomes prepared from electric organ of Electrophorus electricus or cat brain and exposed to ouabain will react with orthophosphate to form a phosphorylated protein. The electrophoretic mobilities of the phosphopeptides cleaved by peptic and Pronase digestion of the microsomes after interaction with phosphate are indistinguishable from those previously known to be formed from microsomes and ...

The conversion of 3-enolpyruvylshikimate .5-phosphate (I) to phenylpyruvate was studied in extracts of Escherichia coli. A separation and partial purification was achieved of the two enzymic activities concerned: chorismate synthetase which catalyzed the 1,4-conjugate elimination (E2’) of orthophosphate from I to yield chorismic acid (II), and the enzyme complex catalyzing the reactions chorism...

Using 32P, uptake and transfer of phosphorus in the microbial food web were studied in surface water from Villefranche Bay (northwestern Mediterranean) from September to December 2001. During the study, the thermocline gradually declined and vertical mixing started, leading to a transition from a nutrient-depleted period to a nutrient-replete period. Before vertical mixing started, the orthopho...

1. The orthophosphate inhibition of photosynthesis by isolated spinach chloroplasts can be reversed by 3-phosphoglycerate, dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, fructose 6-phosphate and fructose 1,6-diphosphate. 2. Metabolically related compounds such as ribulose 1,5-diphosphate, glucose 6-phosphate, 6-phosphogluconate and phosphoenolpyruvate are ineffective. 3. The kinetics o...

Arsenate does this because the enzyme accepts it as orthophosphate, but the first product breaks down because of the instability of esters and anhydrides of arsenate (cf. Braunstein, 1931). This use of arsenate is limited because most of the biochemical reactions that liberate orthophosphate, in contrast with those that liberate pyrophosphate, are not freely reversible. Arsenical analogues of p...

One-dimensional stray field (STRAFI) profiles of 31P in phantoms containing, variously, calcium hydroxyapatite, calcium orthophosphate and cobaltous phosphate octahydrate were obtained in a STRAFI field of 11.7 T on a 19.6 T magnet at 206 MHz. The intrinsic spatial resolution with the apatite and orthophosphate samples was about 100 μm. The profile of the image from the paramagnetic cobaltous s...

Microsomes prepared from electric organ of Electrophorus electricus or cat brain and exposed to ouabain will react with orthophosphate to form a phosphorylated protein. The electrophoretic mobilities of the phosphopeptides cleaved by peptic and Pronase digestion of the microsomes after interaction with phosphate are indistinguishable from those previously known to be formed from microsomes and ...

Results from different research suggest that metallic iron (Fe0) materials enhance anaerobic digestion (AD) systems to remove organics (chemical oxygen demand (COD)), phosphorus and nitrogen polluted water. However, the available results are difficult compare because they derived experimental conditions. This characterises effects of Fe0 type dosage in AD simultaneously COD nutrients (orthophos...

The rapid growth of aquaculture is accompanied by increased discharge of nutrient-rich wastewaters into rivers and coastal waters leading to eutrophication and deterioration of water quality. Seaweeds are suitable candidates to reduce dissolved inorganic nutrient concentration discharged through aquaculture effluent, and can improve water quality and allow for sustainable aquaculture. In this s...