Stored red blood cells (RBCs) undergo oxidative stress that induces deleterious metabolic, structural, biochemical, and molecular changes collectively referred to as "storage lesions". We hypothesized that vitamin C (VitC, reduced or oxidized) would reduce red cell storage lesions, thus prolonging their storage duration. Whole-blood-derived, leuko-reduced, SAGM (saline-adenine-glucose-mannitol)...

Hereditary spherocytosis is a genetically determined abnormality of red blood cells. It is likely that a defect of intracellular glycolysis results in the formation of spherical cells (spherocytes) which have a reduced life span and exhibit increased osmotic fragility in hypotonic saline solutions, increased susceptibility to mechanical trauma, and spontaneous lysis on incubation. Lysis is mark...

The effect of parathyroid hormone on erythrocytes from newborn and adult rabbits was studied in relation to the fragility pattern in hypotonic salt solutions and the activities of Ca- and Mg-dependent ATPases. Median osmotic fragility of red blood cells from newborn rabbits was significantly higher than in red blood cells from mature rabbits. Parathyroid hormone increased the mean osmotic fragi...

The biomechanics of erythrocytes, determined by the membrane integrity and cytoskeletal structure, provides critical information on diseases such as diabetes mellitus, myocardial infarction, hypertension, and sickle cell anemia. Here we demonstrate a simple microfluidic tool for examining erythrocyte fragility based on characterizing osmotic lysis kinetics. Hydrodynamic focusing is used for gen...

BACKGROUND The main problem of tremor is the damage caused to the quality of the life of patients, especially those at more advanced ages. There is not a consensus yet about the origins of this disorder, but it can be examined in the correlations between the biological signs of aging and the tremor characteristics. METHODS This work sought correlations between the osmotic fragility of erythro...

The effects of O33 and O49 P. mirabilis lipopolysaccharides (LPSs) on human erythrocyte membrane properties were examined. Physical parameters of the plasma membrane, such as membrane lipid fluidity, physical state of membrane proteins, and osmotic fragility, were determined. The fluidity of the lipids was estimated using three spin-labeled stearic acids of doxyl derivatives: 5-doxylstearic aci...

Two adult cases of thrombohaemolytic thrombocytopenic purpura are described. Both showed striking morphological changes in the red cells with increased saline osmotic fragility, in addition to thrombocytopenia and generalized hyaline thrombi affecting small blood vessels. These changes support the hypothesis that this is a triple auto-immune disorder affecting red cells, platelets, and small bl...