To evaluate whether duration of autologous oocyte vitrification has an impact on in-vitro fertilization (IVF) cumulative live birth rates (cLBR) after fresh or frozen embryo transfers. We identified IVF cycles using vitrified/thawed oocytes from 2010-2020. Ovarian stimulation, vitrification/thawing, IVF/ICSI, culture/transfer were performed published protocols. Duration cryopreservation for the...

Ice easily recrystallizes during warming after vitrification, and antifreeze protein (AFP) can inhibit the re-crystallization. However, no study has evaluated the effect of AFP treatment only thereon during warming. This study sought to compare AFP treatment protocols: a conventional protocol with AFP treatment during vitrification and first-step warming and a new protocol with AFP treatment du...

ARTICLES DISCUSSED: Ponthier, J., de la Rebière, G., Salciccia, A., Deleuze, S. Endoscopy guided photoablation of endometrial cysts using a 980 nm laser with contact fiber in mares. Journal Visualized Experiments: JoVE. (161), e61569 (2020). Colombo, M., Luvoni, G. C. Minimum volume vitrification immature feline oocytes. (160), e61523 Barros, R. Lodde, V., Franciosi, F., Luciano, A. M. In vitro...

Cryopreservation of embryos and gametes has become an indispensible service for infertility treatment. Vitrification is now a prevalent technique for cryopreservation in assisted reproductive technology (ART) replacing, in many cases, conventional slow cooling methodology. Fertility preservation in female patients has become a very topical issue. Various malignant and nonmalignant diseases have...

BACKGROUND One of the most major obstacles of ovarian tissue vitrification is suboptimal developmental competence of follicles. Matrix metalloproteinases 2 (MMP-2) and 9 (MMP-9) and their tissue inhibitors TIMP-1 and TIMP-2 are involved in the remodeling of the extracellular matrix in the ovaries. OBJECTIVE This study aimed to evaluate the expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 genes ...

Abstract Background After ovarian tissue transplantation, ischemia-reperfusion injury and free radicals cause follicle depletion apoptosis. Therefore, the use of antioxidants to reduce production is an important method address consequences injury. Resveratrol a natural active polyphenol compound with anti-inflammatory, antitumor, strong antioxidant anti-free radical properties. The aim this stu...