Leptin exerts many biological functions, such as in metabolism and reproduction, through binding to and activating the leptin receptor, LepRb, which is expressed in many regions of the brain. To better understand the roles of LepR downstream signaling pathways, Y123F mice, which expressed mutant leptin receptors with phenylalanine (F) substituted for three tyrosines (Y) (Tyr985, Tyr1077 and Tyr...

BACKGROUND The purpose of this study was to investigate the in vitro survival and developmental potential of oocytes obtained from vitrified mouse ovaries transplanted to a heterotopic site. MATERIALS AND METHODS In this experimental study, two-week-old mice were unilaterally ovariectomized after anesthesia. The ovaries were vitrified by cryotop. After two weeks, the ovaries were thawed and a...

This study determined the effects of inhibiting vascular endothelial growth factor (VEGF) at follicle selection. Marmosets were given an injection of VEGF antagonist, the VEGF Trap on Day 5 of the follicular phase and ovaries were evaluated on Day 10 or 15. Ovaries from controls were assessed on Day 5 (time of selection), Day 10 (peri-ovulatory) and Day 15 (luteal phase). At Day 10, ovaries of ...

Sex-related genes expressed in vitellogenic ovaries of the giant tiger shrimp, Penaeus monodon, were identified by an EST approach. A total of 1051 clones were unidirectionally sequenced from the 5 terminus. Nucleotide sequences of 743 EST (70.7%) significantly matched known genes previously deposited in the GenBank (E-value <10(-4)) whereas 308 ESTs (29.3%) were regarded as newly unidentified ...

Two experiments were undertaken to investigate the cause of the observed tendency of Mongolian gerbil dams to gestate more male than female fetuses in their right uterine horns and more female than male fetuses in their left uterine horns. It was found in Expt 1 that female gerbils that had both ovaries removed and portions of their right ovary placed in both ovarian capsules gestated significa...

The aim of this study is to present the PET/CT findings of surgically transposed ovaries. PET/CT studies and associated abdominal imaging studies of seven women, aged 28-43 years, with 11 transposed ovaries were retrospectively reviewed. Attention was directed to the location and the 18F-Fluorodeoxyglucose (FDG) avidity of the transposed ovaries. On the CT part of the PET/CT, location of the tr...

Mouse fetal ovaries were cultured to investigate germ cell development in the presence of a combination of the growth factors (GFs) stem cell factor, insulin-like growth factor-1 and leukaemia inhibitory factor. Ovaries were isolated from fetal mice at 13 and 14 days post-coitum (dpc) and cultured to the equivalent of 17 dpc. Culture conditions comprised minimal essential medium-alpha plus 5% f...

BACKGROUND The interaction between follicular cells and oocyte leads to a change in gene expression involved in oocyte maturation processes. OBJECTIVE The purpose of this study was to quantify the expression of more common genes involved in follicular growth and oocyte developmental competence. MATERIALS AND METHODS In this experimental study, the expression of genes was evaluated with qRT-...

Introduction: Transposition of the ovaries outside of the pelvis to protect them from pelvic radiation was initially described in 1958. The procedure is indicated in patients diagnosed with malignancies that require pelvic radiation, but not removal of the ovaries, as part of their treatment. It can be performed by laparotomy or laparoscopy, depending on gynecologist’s surgical skill. The aim o...