As opposed to the vast majority of prokaryotic repressors, the immunity repressor of temperate Escherichia coli phage P2 (C) recognizes non-palindromic direct repeats of DNA rather than inverted repeats. We have determined the crystal structure of P2 C at 1.8 Å. This constitutes the first structure solved from the family of C proteins from P2-like bacteriophages. The structure reveals that the ...

BACKGROUND Catheter hub decontamination requires a thorough scrub and compliance varies. This study evaluates the effectiveness of a disinfection cap with 70% alcohol in preventing contamination/infection. METHODS A 3-phased, multifacility, quasi-experimental study of adult patients with central lines divided into P1 (baseline), when the standard scrub was used; P2, when the cap was used on a...

Rabbits were made anemic to different extents by phenylhydrazine injections so as to vary the developmental stages of the erythroid cells in their peripheral blood and bone marrow. It was found that the more severe the anemia, the lower the concentration of glycerate-2,3-P2 in the bone marrow cells and in the circulating erythroid cells. The glycerate-2,3-P2 level was shown to rise during eryth...

Phosphoinositides, particularly phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3] and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], are recognized by SHIP2 (also known as INPPL1) a member of the inositol polyphosphate 5-phosphatase family. SHIP2 dephosphorylates PI(3,4,5)P3 to form PI(3,4)P2; the latter interacts with specific target proteins (e.g. lamellipodin). Although the prefer...

The complete genome sequences of pepper mild mottle virus (PMMoV)-P2 and -P3 were determined by the Sanger sequencing method. Although PMMoV-P2 and PMMoV-P3 have different pathogenicity in some pepper cultivars, the complete genome sequences of PMMoV-P2 and -P3 are composed of 6,356 nucleotides (nt). In this study, we report the complete genome sequences and genome organization of PMMoV-P2 and ...

There exists a balanced ternary design with block size 3 and index 2 on 2v P2 + 4 and 2v P2 + 1 elements with a hole of size v, for all positive integers v and P2, such that v ~ 2P2 + 1. As an application of this result, we determine the numbers of common triples in two simple balanced ternary designs with block size 3 and index 2, for P2 = 3 and 4.

The C-repressor of bacteriophage P2 (P2 C) is a DNAbinding protein that controls the lifecycle of the P2 bacteriophage by directing it toward the lysogenic mode. P2 C is a 99 amino acids protein, which forms stable homodimers but not higher oligomers in the absence of DNA (Ahlgren-Berg et al. 2007). As opposed to the more common situation where dimeric proteins bind palindromic DNA-sequences, P...

Phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) synthesis has been implicated in maintaining the function of the Golgi apparatus. Here we demonstrate that the inhibition of PtdIns(4,5)P2 synthesis in vitro in response to primary alcohol treatment and the kinetics of Golgi fragmentation in vivo were very rapid and tightly coupled. Preloading Golgi membranes with short chain phosphatidic ac...

When we know the subjective probabilities (degrees of belief) p1 and p2 of two statements S1 and S2 , and we have no information about the relationship between these statements, then the probability of S1 & S2 can take any value from the interval max(p1 + p2 ? 1; 0); min(p1; p2)]. If we must select a single number from this interval, the natural idea is to take its midpoint. The corresponding \...

A property of graphs is any isomorphism closed class of simple graphs. For given properties of graphs P1,P2, . . . ,Pn a vertex (P1,P2, . . . ,Pn)-partition of a graph G is a partition {V1, V2, . . . , Vn} of V (G) such that for each i = 1, 2, . . . , n the induced subgraph G[Vi] has property Pi. The class of all graphs having a vertex (P1,P2, . . . ,Pn)partition is denoted by P1◦P2◦ · · · ◦Pn....