Viral attacks on natural populations of herbaceous forage legumes might have adverse effect on seed and herbage production, hence this study was carried out to investigate the type and incidence of viruses occurring on these plants in Nigeria. Protein A-sandwich (PAS) and antigen-coated plate (ACP) enzyme-linked immunosorbent assays (ELISA) were used to detect ten legume viruses from 100 leaf s...

It is often claimed that programmed cell death (pcd) exists in plants and that a form of pcd known as the hypersensitive response is triggered as a defense mechanism by microbial pathogens. However, in contrast to animals, no feature in plants universally identifies or defines pcd. We have looked for a hallmark of pcd in animal cells, namely, DNA cleavage, in plant cells killed by infection wit...

Prepuberal (P) gilts were induced to ovulate with pregnant mare serum gonadotropin followed 72 h later by human chorionic gonadotropin (hCG). Three P gilts and three mature (M) gilts each were ovariectomized on d 10, 14, 18, 22 and 26 (d 0 = day of hCG for P gilts and onset of estrus for M gilts). Gilts ovariectomized on d 14, 18, 22 and 26 were hysterectomized on d 6 to ensure maintenance of t...

BACKGROUND Cowpeas are important staple legumes among the rural poor in northern Ghana. Our objectives were to assess the iron and zinc content of cowpea landraces and identify factors that predict the intention of mothers/caregivers to give cowpeas to their schoolchildren. METHODS AND FINDINGS We performed biochemical analysis on 14 landraces of cowpeas and assessed the opinion of 120 caregi...

In the current study, response surface methodology (RSM) was used to assess the effects of extraction time and temperature on the content of bioactive compounds and antioxidant activity of purple basil leaf (Ocimum basilicum L.) extracts. The stability of anthocyanins in relation to temperature, light and copigmentation was also studied. The highest anthocyanin content was 67.40 mg/100 g extrac...

An endogenous cowpea mosaic virus (CPMV) RNA-protein complex (CPMV replication complex) capable of elongating in vitro preexisting nascent chains to full-length viral RNAs has been solubilized from the membrane fraction of CPMV-infected cowpea leaves using Triton X-100 and purified by Sepharose 2B chromatography and glycerol gradient centrifugation in the presence of Triton X-100. Analysis of t...

A field experiment was carried out to investigate the effects of variety and spatial arrangement on pest incidence, damage and subsequent yield of cowpea in a cowpea/maize intercrop. Two varieties of cowpea (Tvu 13076 and Ife brown) and two spatial arrangements (2:3 maize/ cowpea & 1:1maize/ cowpea intercrops) were investigated. There was Lower population density of Maruca vitrata among the 1:1...

The study was conducted to improve cocoyam-based recipes (steamed cocoyam paste [ebiripo], ikokore, and fried cocoyam balls [ojojo]) using different blends of cocoyam and cowpea flours (100:0, 80:20, 70:30, 60:40, and 50:50). The proximate composition, mineral composition, and sensory qualities of the recipes were determined using standard analytical procedures. The recipes had significantly (P...

An experiment was undertaken at the College of Agriculture, Vellayani, Kerala to study effect organic nutrition on growth attributes and yield taro during 2019-20 2020-21. The treatments comprised six levels sources (S1 - FYM + wood ash; S2 ash PGPR mix I; S3 I vermiwash; S4 Poultry manure S5 S6 vermiwash) two in-situ green manuring (G1 with cowpea; G2 dhaincha ) three controls (C1 Nutrient man...