Pullulanase from Bacillus acidopullulyticus was purified on a packed bed and a fluidized bed of calcium alginate beads. The binding of enzyme activity to the medium was found to follow Langmuir isotherm pattern. The maximum binding capacity was 1476 U ml(-1) matrix and the dissociation constant was 142 U ml(-1). The dynamic binding capacities at 5% breakthrough in the packed and fluidized beds ...

The discharge of cyanide-containing effluents into the environment contaminates water bodies and soil. Effective methods of treatment which can detoxify cyanide are the need of the hour. The aim of the present study is to develop a bioreactor for complete degradation of cyanide using immobilized cells of Serratia marcescens RL2b. Alginate-entrapped cells of S. marcescens RL2b were used for comp...

The Biosorption property of S. cerevisiae for chromium uptake was investigated in an immobilized cell bioreactor. Saw dust was utilized as the solid bed in the reactor. Adsorption of S. cerevisiae on saw dust obeys a first order reaction kinetic up to 6 hours. The immobilized biomass particles are porous and exist in the new generation of biological adsorbent. Chromium biosorption was studied i...

In this paper a packed bed of char particles is considered for experimental study and analysis. The packed char bed is modelled by extending the single-particle analysis (Dasappa et al., 1994a, Chem. Eng. Sci. 49}2: 223}232. Dasappa et al., 1994b, 1wenty-,fth Symposium (International) on Combustion, pp. 1619}1628. Dasappa et al., 1998, 1wenty-seventh Symposium (International) on Combustion, pp....

Background and experimental approach A main challenge in cell therapies and other tissue regeneration approaches is to produce a therapeutically significant cell number. For expansion of mesenchymal stem cells (MSC) the cultivation on 2D plastic surfaces is still the conventional procedure, even though the culture conditions differ significantly from the 3D environment in vivo. Additionally, st...

Electrospun nanofiber membranes surface functionalized with 3D nanolayers through ATRP provide adsorption capacities over 50-times higher than current commercial membrane adsorption systems and over 12-times higher than packed bed resins; additionally, the adsorption kinetics remain 10-times faster than packed bed resins and have over 15-times higher permeance.

Expanded Bed Adsorption enables the protein recovery directly from cultivations of microorganisms or cells and preparations of disrupted cells, without the need for prior removal of suspended solids. The performance ofan expanded bed is comparable to a packed bed owing to reduced mixing of the adsorbent particles, clogging/plugging of solid particles in the column, while fluidized bed requires ...