Adenosine 3’,5’-monophosphate (3’,5’-AMP) was assayed in calvaria isolated from fetal rats at term. A marked increase in 3’,5’-AMP was detected in vitro within 1 min after adding purified parathyroid hormone to calvaria incubated in Krebs-Ringer-bicarbonate or Krebs-Ringer-Tris buffer. A maximal effect was detected after 5 min of incubation with added parathyroid hormone. The lowest concentrati...

Secondary hyperparathyroidism in pseudohypoparathyroidism was suggested by Kolb (1962) based mainly on bone X-ray findings characteristic to parathyroid hyperfunction such as subperiosteal resorption and osteitis fibrosa. We have recently experienced a case of typical pseudohypoparathyroidism in which the parathyroid hormone-like calcium-mobilizing activity in urine was increased, although bone...

Kidney transplantation (KT) can reverse renal function and improve patient survival while intact parathyroid hormone (iPTH) level persists high in some kidney transplant recipients (KTRs). Thus we examined the prevalence associated factors of persistent hyperparathyroidism (pHPT).

The standard measurement of parathyroid hormone (PTH) is the intact PTH (iPTH) assay, which is used for approximately 90% of Japanese dialysis patients. The iPTH assay reacts not only with 1-84 PTH, but also with large truncated fragments of non-1-84 PTH, including 7-84 PTH. On the other hand, the whole PTH assay is specific for 1-84 PTH. The aim of the current study was to define the validity ...

Glucocorticoids are known to affect calcium balance but not thought cause clinically significant hypocalcemia. This case adds a list of isolated reports noting hypocalcemia developing post administration glucocorticoids in patient with hypoparathyroidism, postulating parathyroid hormone deficiency as risk factor decompensated homeostasis.

Relations between platelet cytosolic calcium, parathyroid hormone, and blood pressure were investigated in 91 normotensive subjects: 47 men and 44 women ranging in age from 24 to 70 years. The men had higher mean arterial blood pressure, serum creatinine, and body mass index than the women. Serum total calcium, plasma ionized calcium, and parathyroid hormone (measured as both intact hormone and...

The determination of intact parathyroid hormone levels is used for diagnosis and in the management of renal osteodystrophy. Pre-analytical and analytical conditions are important in the overall confidence of the assay. Unfortunately, there are no clear recommendations for the use of serum samples or samples anticoagulated with ethylenediaminotetraacetic acid (EDTA) for the best preservation of ...

References 1. Glendenning P, Gutteridge DH, Retallack RW, Stuckey BG, Kermode DG, Kent GN. High prevalence of normal total calcium and intact PTH in 60 patients with proven primary hyperparathyroidism: a challenge to current diagnostic criteria. Aust N Z J Med 1998;28:173–8. 2. Vasikaran SD, Sturdy G, Musk AA, Flicker L. Vitamin D insufficiency and hyperparathyroidism in Perth blood donors. Med...

Immunohistochemical staining for parathyroid hormone-related protein was performed in 27 tumours from 19 normocalcaemic and eight hypercalcaemic patients with cancer. All the tumours from hypercalcaemic patients stained positively for the protein, as did 17 tumours from normocalcaemic patients. Only hypercalcaemic patients had biochemical evidence of increased bone resorption and abnormalities ...

Parathyroid adenoma is a benign tumor of the parathyroid glands. The cause of most parathyroid adenomas is unknown. Parathyroid adenoma increases the secretion of parathyroid hormone and results in primary hyperparathyroidism. High amounts of parathyroid hormone in the blood cause the imbalance of calcium, which leads to various complications such as kidney stones, depression, lethargy, nausea,...