The aim of this study was to determine possible differences in the faecal microbiota of dairy cows infected with Mycobacterium avium subsp. paratuberculosis (Johne's disease) in comparison with noninfected cows from the same herds. Faecal samples from cows in 4 herds were tested for M. avium subsp. paratuberculosis by real-time PCR, and faecal bacterial populations were analysed by 454 pyrosequ...

Paratuberculosis (Johne’s disease) is an OIE listed notifi able economically important contagious mycobacterial disease of ruminants caused by Mycobacterium avium subspecies paratuberculosis. The present study reports the seroprevalence of paratuberculosis in bovines of the rural area from fi ve different agro-climatic zone of Punjab. A total of 736 serum samples from both cattle and buffalo he...

to investigate the prevalence of mycobacterium paratuberculosis (m. avium subsp. paratuberculosis)infection among cattle slaughtered in ahvaz abattoir, samples were taken from 250 cattle. before slaughter,sex and age were assessed. thereafter, samples were taken from ileocecal valve, rectum mucosa, ileocecallymph node and stool. all smears which taken from these samples were stained by ziehl-ne...

We read with interest the report of Sechi et al. (1) on the associations among Crohn’s disease (CD), Mycobacterium avium subsp. paratuberculosis, and carriage of mutant alleles of the NOD2/CARD15 gene. We believe that the study by Sechi et al. was well conceived, is critical to understanding the etiology of Crohn’s disease, and should stimulate additional interest in the field. Unfortunately, t...

Crohn’s disease in humans has strong similarities to Johne’s disease in cattle, but with a poorly defined etiology. Mycobacterium paratuberculosis, the causative agent of Johne’s disease, has been linked as a causal agent of Crohn’s disease in humans. However, evidence to date does not prove that Crohn’s disease is caused by M. paratuberculosis and may, in fact, be a multifactorial disorder. Re...

Paratuberculosis is a chronic bowel disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Its secretion through semen highlights the importance of paratuberculosis-free breeding bulls. The breeding and teaser bulls at three semen production units (SPUs) located in Punjab, Pakistan, were screened for the presence of antibodies against MAP. A total of 253 samples were ...

UV light inactivation of Mycobacterium avium subsp. paratuberculosis in Middlebrook 7H9 broth and whole and semiskim milk was investigated using a laboratory-scale UV machine that incorporated static mixers within UV-penetrable pipes. UV treatment proved to be less effective in killing M. avium subsp. paratuberculosis suspended in milk (0.5- to 1.0-log(10) reduction per 1,000 mJ/ml) than that s...

The PTB-Simherd is a dynamic, stochastic, and mechanistic Monte-Carlo model simulating adairy herd including young stock. Paratuberculosis and relevant control strategies against thisdisease was built into an existing simulation model. The model is able to simulate bothepidemiological, technical and economic consequences of paratuberculosis and controlstrategies against it in th...

Conflicting reports on the heat resistance of Mycobacterium paratuberculosis prompted an examination of the effect of culture medium on this property of the organism. M. paratuberculosis was cultured in three types of media (fatty acid-containing medium 7H9-OADC (oleic acid-albumin-dextrose-catalase supplement) and glycerol-containing media WR-GD and 7H9-GD [glycerol-dextrose supplement]) at pH...