Autotransporters constitute the biggest group of secreted proteins in Gram-negative bacteria and contain a membrane-bound beta-domain and a passenger domain secreted to the extracellular environment via an unusually long N-terminal sequence. Several passenger domains are known to be glycosylated by cytosolic glycosyl transferases, promoting bacterial attachment to mammalian cells. In the presen...

Autotransporter (AT) proteins are a large and diverse family of extracellular virulence proteins from Gram-negative bacteria, characterized by a central beta-helix domain within the mature virulence protein. It is not clear how these proteins cross the outer membrane (OM) quickly and efficiently, without assistance from an external energy source such as ATP or a proton gradient. Conflicting res...

Bus hub is characterized with multiple berths, each serving specific bus routes. When routes at different berths share the same stops en route, passengers to shared may rush among these catch newly arriving bus, causing inconvenience and deteriorating service level. Thus, this research attempts optimize route assignment a minimize passenger transfer distance optional berths. The problem modeled...

A large number of passenger information systems for public passenger transport exist in Europe at national level but their trans-national interoperability is very limited. A passenger information system depends on many underlying heterogeneous information systems; ranging from management of transport infrastructure network, public transport infrastructure objects, public passenger transport ser...

The protein McaP was previously shown to be an adhesin expressed by the Moraxella catarrhalis strain O35E, which also displays esterase and phospholipase B activities (J. M. Timpe et al., Infect. Immun. 71:4341-4350, 2003). In the present study, sequence analysis suggests that McaP is a conventional autotransporter protein that contains a 12-stranded beta-barrel transporter module (amino acids ...

The flow of departing passengers from the airport inside terminal is affected by strategic and tactical decisions operation teams. made managers regarding opening closing times check-in, boarding, gates are critical to optimize performance operation. In this research, Izmir Adnan Menderes Airport was taken as a use case.Departure building security control, boarding focus entities.The simulation...

Public transportation has significant role to regulates human mobility be effective. But, people in Belinyu low interest towards public transportation. is now decreasing and the malfunction terminal cause appearance of phantom terminal. This research analyze sustainability Belinyu. The data was obtain from distribution questionnaire passengers drivers, interview for owner company, District Offi...

We consider the optimal scheduling of a nite capacity shuttle in a two node network with imperfect information. When shuttle trips do not depend on the number of passengers carried, we prove optimality and monotonicity of threshold policies. We derive conditions for dispatching which reduce the computational eeort required to compute an optimal threshold policy. We provide a counterexample to t...