Small molecules that interact with DNA are known to be effective as anticancer and antimicrobial agents. Therefore it is significant search for new interacting potential therapeutic In this study, we aimed investigate interactions of novel fluorine substituted imine compound DNA, (E)-2-((3,5-bis(trifluoromethyl) phenylimino)methyl)-4,6-dimethoxyphenol, its biological activities. the were invest...

We have determined that the type II restriction enzyme Ava II, isolated from Anabaena variabilis, recognizes and cuts the sequence (formula: see article). The eight Ava II sites of pBR322 have been mapped, as well as a unique site for Ava I.

Calcium chloride is commonly included in buffers used to generate chemocompetent bacterial cells. The mechanism that underlies the uptake of exogenous DNA into bacteria is not completely understood. Previous studies have suggested that calcium ions may function to neutralize electrostatic repulsion between DNA and membrane proteins, thereby allowing exogenous DNA to enter membrane pores. Other ...