DNA vaccination against Foot-and-Mouth Disease Virus (FMDV) is an attractive and alternative strategy to the use of classical inactivated viral vaccines. The injection of a pcDNA3.1-based DNA vaccine encoding for FMDV P1-2A3C3D and GM-CSF proteins had previously been shown to induce the production of neutralizing antibodies against FMDV and partially protect swine against an experimental challe...

Plasmid DNA chromatography is a powerful field in constant development and evolution. The use of this technique considered mandatory the production an efficient safe formulation to be applied for plasmid-mediated gene therapy. Concerning this, search ideal chromatographic support/ligand combination motivated scientist pursue continuous improvement on plasmid performance, looking progression lig...

background and objectives: bacteria, most prevalently the pseudomonas species possess high capacity to utilize and de- grade petroleum hydrocarbons and are classified as the hydrocarbonoclastic microorganisms. many species of the genus pseudomonas are notorious for their aerobic degradation capacity, extracellular enzyme production and are metabolically versatile organisms capable of utilizing ...

The disruption of microvascular barrier in response to advanced glycation end products (AGEs) stimulation contributes to vasculopathy associated with diabetes mellitus. Here, to study the role of Src and its association with moesin, VE-cadherin and focal adhesion kinase (FAK) in AGE-induced vascular hyperpermeability, we verified that AGE induced phosphorylation of Src, causing increased permea...

Anandamide amidase is the hydrolytic enzyme responsible for the breakdown of anandamide, an endogenous cannabimimetic, to arachidonate and ethanolamine. Another enzymatic activity called anandamide synthase catalyzes the reverse reaction, that is the condensation of arachidonate and ethanolamine. Using a recently cloned rat fatty acid amidohydrolase (FAAH), we tested the hypothesis that the syn...

Calcium phosphate nanoparticles (CaP-NP) are ideal tools for transfection due to their high biocompatibility and easy biodegradability. After transfection these particles dissociate into calcium and phosphate ions, i.e. physiological components found in every cell, and it has been shown that the small increase in intracellular calcium level does not affect cell viability. CaP-NP functionalized ...

1 Animal Scientist, D.Sc., Adjunct Professor, Department of Animal Science, Universidade Federal de Viçosa, ViçosaMG, 36571-000, CNPq and INCT-Ciência Animal researcher ([email protected]) 2 Animal Scientist, D.Sc., Titular Professor, Department of Animal Science, Universidade Federal de Viçosa, Viçosa-MG, Brazil, 36571-000, CNPq and INCT-Ciência Animal researcher ([email protected]) 3 Agronomy Engi...