Brucellosis is a widespread zoonosis. Currently the diagnosis of this zoonosis is based on microbiological and serological laboratory tests. Polymerase chain reaction (PCR) has been used to detect DNA from Brucella. Different target genes, primer pairs, PCR techniques, and extraction procedures have previously been published for Brucella detection. But only a few of these primers have been used...

Background: As a novel pro-apoptotic kinase, Mammalian sterile 20–like kinase 1 (Mst1) promotes programmed cell death in animal models of inflammatory diseases. This research aimed to determine the level of expression of Mst1 gene in a rat model of spinal cord injury (SCI) treated with valproic acid (VPA). Methods: Animals were divided into four groups: Contused animals (untreated); laminectom...

The red sea bream iridovirus (RSIV) belonging to genus Megalocytivirus is responsible for iridoviral disease (RSIVD) in marine and freshwater fishes. Although several diagnostic assays RSIV have been developed, sensitivity (DSe) specificity (DSp) of real-time polymerase chain reaction (PCR) are not yet evaluated. In this study, we developed a TaqMan probe-based PCR method evaluated its DSe DSp....

conclusions results of the present study showed that the tst and sea genes had high frequencies among the mrsa isolates. the increased prevalence of mrsa isolates containing different virulence genes, probably accompanied by antimicrobial resistance, can complicate the therapy of mrsa isolates. results all the tested isolates were susceptible to vancomycin, but resistant to penicillin (100%), e...

milk is considered a nutritious food because it contains several important nutrients including proteins and vitamins. conversely, it can be a vehicle for several pathogenic bacteria such as staphylococcus aureus. this study aimed to analyze the frequency of genes encoding the nine staphylococcal enterotoxins (ses) and enterotoxin gene profiles in s. aureus isolates derived from raw bovine milk....

Introduction: Pseudomonas aeruginosa is a serious challenge for antimicrobial therapy, due to chromosomal mutations or intrinsic resistance to various antimicrobial agents, such as Metallo-β-Lactams (MBL). This study aimed to investigate the prevalence of β-lactamases encoding genes among P. aeruginosa strains isolated from intensive care unit (ICU) patients by phenotypic and multiplex PCR meth...

Purpose: To develop a multiplex-urease PCR assay specific for ureA and ureB genes directly from gastric biopsy specimens and compare the results of multiplex-urease PCR assay with rapid urease test (RUT) and histopathology. Methodology: This study was conducted on 109 patients. A RUT was run; histopathological staining and multiplex urease PCR were applied to biopsy specimen to detect H. pylori...

Cytomegalovirus is the most important pathogen affecting transplant recipients and causing significant morbidity and morbidity. The prevalence of CMV  infections in transplant recipient varies from country to country.  The aim of this study was to determine the prognostic value of PCR assay for CMV  detection in patients before Keratoplasty.  A total of 23 patient samples en...