نتایج جستجو برای: pcr polymeras chain readins
تعداد نتایج: 433273 فیلتر نتایج به سال:
abrin known as ribosome inactivating protein’s (rip) it is a high cytotoxic plant protein. high lethality and low cost and easy access to plant and seeds lead to this toxin used in crimes and terrorist acts. because obtaining purified toxins requires advanced laboratory equipment and complex procedures, so, it appears that the perpetrators of such crimes use crude extracts. as a result, remaini...
The current study's goal was to identify virulence factors (genes) in P. mirabilis. A total of 25/100 (25%) Proteus mirabilis were obtained from patients with otitis media and identified using culture biochemical characteristics, Polymerase chain reaction (PCR). PCR technique used detect the 16S rRNA gene all factors, including ureC gene, which encodes urease synthesis, flaA flagella fimbriae p...
BACKGROUND We describe the development and validation of a new quantitative real time PCR (qrt-PCR) method for the enumeration of the toxic benthic dinoflagellate Ostreopsis cf. ovata in marine environment. The benthic Ostreopsis sp. has a world-wide distribution and is associated during high biomass proliferation with the production of potent palytoxin-like compounds affecting human health and...
BACKGROUND Few studies have addressed the timing of cervical cytologic abnormalities and human papillomavirus (HPV) positivity during the course of an infection. It remains largely unknown how infections detected by HPV and cytology wax and wane relative to each other. The aim of this analysis was to assess the longitudinal relationship of abnormal cytology and HPV positivity in a 7-year prospe...
BACKGROUND Recently, Stein et al. identified the metastasis-associated in colon cancer 1 (MACC1) gene by genome-wide search for differentially expressed genes in human colon cancer tissues and metastases. PATIENTS AND METHODS We analyzed MACC1 expression levels in 52 colorectal cancer samples using quantitative real-time polymerase chain reaction (QRT-PCR). RESULTS We found that MACC1 expre...
AIM To investigate the dose-response effect of humic acid (HA) on the quantitative real time polymerase chain reaction (QRT-PCR) inhibition and the efficiency of Taq polymerase increment in preventing inhibition by HA in DNA extracted from ancient bones. METHODS DNA was isolated from bone samples and DNA quantification was conducted with the real-time 5' exonuclease detection assay (TaqMan), ...
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