OBJECTIVE An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness. METHODS In previous work, we constructed a skin cDNA library and isolated a full-length cDNA clone termed KAP11.1. On this basis, we conducted a series of bioinformatics analysis. Tissue distribution of KAP11.1 mRNA was performed using semi-quantitative reverse transcripti...

OBJECTIVE To better detect occult cervical metastases. DESIGN RNA from 153 cervical lymph nodes was analyzed for the presence of squamous cell carcinoma using quantitative cytokeratin (CK) 14 real-time reverse transcription polymerase chain reaction (RT-PCR). Detection of CK RNA in pathologically negative nodes was further analyzed by semi-step sectioning and CK immunohistochemistry. Subjects...

BACKGROUND Congenital toxoplasmosis is associated with variable complications including encephalitis, microcephaly, hydrocephaly, hepatitis, lymphadenopathy and even intrauterine death. Presence of Toxoplasma gondii in human placenta may induce congenital infection. The aim of this study was to determine the genotypes of Toxoplasma gondii infection in human spontaneous aborted fetuses in Shiraz...

Objectives: A multi-site study evaluated the clinical performance of the Roche LightCycler PCR assay in the detection of methicillin-resistant Staphylococcus aureus (MRSA) in comparison to routine chromogenic agar culture method (BD BBL CHROMagar II). Two other variables were also investigated: to establish if screening the throat increases the detection rate of MRSA colonisation; and, to deter...

To assess the clinical relevance of a semi-quantitative measurement of human cytomegalovirus (HCMV) DNA in renal transplant recipients within the typical clinical context of a developing country where virtually 100% of both receptors and donors are seropositive for this virus, we have undertaken HCMV DNA quantification using a simple, semi-quantitative, limiting dilution polymerase chain reacti...

background: theileriosis is a blood protozoan disease with high mortality in cattle in tropical and subtropical regions of the world. several studies were conducted to identify theileria species in infected cattles and vector ticks by microscopic examination. however, microscopic technique has lower sensitivity compared to molecular method.                  objectives: this study was carried ou...

the aim of this study was to identify genes that are predictive of carcinogenic change in patients of oral leukoplakia (olK) using a cancer-related microarray. candidate biomarkers were discovered using the oligo geArray oHs-802 and validated on independent samples by semi-quantitative reverse transcription-pcr (rt-pcr) and real-time rt-pcr. Both the discovery and the validation cohorts of samp...

One important goal of genomics is to explore the extent of alternative splicing in the transcriptome and generate a comprehensive catalog of splice forms. New computational and experimental approaches have led to an increase in the number of predicted alternatively spliced transcripts; however, validation of these predictions has not kept pace. In this work, we systematically explore different ...

Epstein-Barr virus (EBV), a member of the family Herpesviridae, is widely spread in the human population and has the ability to establish lifelong latent infection. In immunocompetent individuals the virus reactivation is usually harmless and unnoticeable. In immunocompromised patients productive infection or type III latency may lead to EBV-associated post-transplant lymphoproliferative disord...