We have adapted a method of amplifying and sequencing genomic DNA (1) for rapidly identifying and partially sequencing genes or transcripts from members of a multi-gene family in which one end is conserved and the other end is highly variable. By utilizing nested, conserved sequence primers in conjunction with primers specific for 6 bp restriction enzyme recognition sequences in a PCR, a family...

An improved protocol is described for using lambda exonuclease to directly sequence PCR products. It is important not to execute PCR cycles beyond the plateau of amplification. The asymmetric PCR and double-stranded DNA sequencing by a snap-cooling procedure were also performed using the same DNA samples and primers. The improved method was the most reliable and produced the best results.

Torque teno virus (TTV) is prevalent worldwide and has been extensively studied in human and some wild and domestic animals. As the studies on TTV in chickens was rare and there was no information about the infection of domestic village chickens with TTV and also structural resemblance of this virus to chicken anemia virus, the frequency of the infection in domestic village chickens in differen...