Gill-associated virus (GAV) is a nidovirus that commonly infects Penaeus monodon (black tiger shrimp) in eastern Australia, causing morbidity and mortalities in the acute stage of disease. Here we explored the possibility of inhibiting GAV replication and disease using double-stranded (ds)RNAs expressed in bacteria and delivered either orally or by muscle injection. To enhance potential RNA int...

A highly sensitive test based on reverse transcription followed by nested polymerase chain reaction (RT-nPCR) was developed to detect the Australian yellow-head-like viruses, gill-associated virus (GAV) and lymphoid organ virus (LOV) of Penaeus monodon. The RT-nPCR detected viral RNA in as little as 10 fg lymphoid organ total RNA isolated from GAV-infected P. monodon. Amplification of serial di...

The effect of exclusion of individual water-soluble (thiamine, riboflavin, pyridoxine, cyanocobalamin, pantothenic acid, folic acid, niacin, biotin, choline, inositol, ascorbic acid) and fat-soluble vitamins (vit. A, D, K and E) in semi-purified diets on growth and survival of juvenile shrimp, P. monodon was studied in the laboratory for 8 weeks. Diets lacking riboflavin and vitamin K did not a...

This work shows an update of the sightings and reports tiger shrimp on Mexican coast Gulf Mexico recorded from 2012 to 2019 smallest specimen found in western Atlantic. The results show increase almost 300% since first report. zone with most was Campeche Yucatan. wide range size distribution captured specimens, including Atlantic presence females at advanced stage gonadal maturity, indicates th...

ABSTRACT: The effect of methods to remove protein content on the properties glucosamine hydrochloride from shells white leg shrimp (Litopenaeus vannamei) and black tiger (Penaeus monodon) was investigated. Chitin obtained by demineralization in 6% HCl for 12h, deproteinization two different (first group soaked 8% NaOH 36h second treated Alcalase enzyme at concentration 0.2% 36h). Two samples we...