The industrial production of penicillin G by Penicillium chrysogenum requires the supplementation of the growth medium with the side chain precursor phenylacetate. The growth of P. chrysogenum with phenylalanine as the sole nitrogen source resulted in the extracellular production of phenylacetate and penicillin G. To analyze this natural pathway for penicillin G production, chemostat cultures w...

A great deal of work has been directed toward increasing the yield of penicillin in fermentations with Penicillium chrysogenum. The spectacular increase in yield which has been achieved has resulted, in the main, from two approaches. In the first approach, mutant strains of P. chrysogenum produced by spontaneous variation or by the action of mutagenic agents such as nitrogen mustard or ultravio...

A soluble, cytochrome linked flavoprotein which oxidizes lactic acid to pyruvic acid has been isolated from Penicillium chrysogenum, strain NRRL 1951 -B25. The reduction of cytochrome c by a flavin enzyme has precedence in the work of Horecker and Heppel (1949) and Mlorell (1952) who have shown that xanthine oxidase can reduce cytochrome c. Morell reported the enzyme to be inhibited strongly by...

The use of glass beads in a high-speed mixing device to rupture organisms was applied to molds. The use of a mixer in which the propellor shaft enters from the top into a metal mixing chamber made it possible to immerse the whole device in a salt water and ice mixture so that the temperature of the glass-bead slurry could be kept below 5 C without difficulty. Mycelia, glass beads, and buffer in...

The inhibitory effects of nisin, natamycin and malic acid, incorporated in whey protein films with pH 3, were investigated alone or with addition of sucrose esters, Tween80 or EDTA. Water vapour permeability measurements and mechanical and rheological tests were also assessed. EDTA and Tween80 did not significantly (P < 0.05) influence the inhibitory activity of films against Pseudomonas aerugi...

The study included the extraction and purification of glucose oxidase enzyme from Penicillium chrysogenum, pure by precipitation with ammonium sulfate at a saturation rate 70% specific activity in this step reached (10.802) units/mg fold 1.276, was followed dialysis step, 13,593 48.184. Finally, ion exchange chromatography using DEAE-Cellulose column, 19.355 units/mg, 36.59, after which migrate...

Compounds structurally related to lysine were tested against Penicillium chrysogenum Wis. 54-1255 for inhibition of growth, sporulation, and penicillin formation. This strain is relatively resistant to lysine analogs. The compounds that were the more active inhibitors of growth and whose activities were reversed by L-lysine were diaminohexynoic acid, N-epsilon-methyllysine, N-alpha-methyllysine...

School buildings typically serve a number of functions simultaneously, owing to which they seem to be especially exposed to the expansion of fungi with wide enzymatic capabilities, including fungi of the genus Penicillium. In total, 34 isolates belonging to 20 species were obtained within two schools. The most frequently isolated species included: Penicillium chrysogenum, P. waksmanii, and P. c...