In this study, complete purification, characterization, and immobilization of polygalacturonase (pectinase) from Penicillium notatum were carried out to achieve an economical suitable alternative for industrial fruit juice clarification. Biosynthesis was under pre-optimized conditions employing solid-state fermentation at a pilot scale using wheat bran. The enzyme subjected series steps purific...

Much att,ention has been given to t,he possibility that desthiobiotin is a precursor of biotin in biological systems, since Melville, Dittmer, Brown, and du Vigneaud (1) first reported that desthiobiot.in was equally as effective as biot,in in stimulating the gr0wt.h of Saccharomyces cemvisiae. Dittmer, Melville, and du Vigneaud (2) later demonst,rat,ed that desthiobiotin was converted by yeast...

Fermentation studies on Penicillium notatum in surface cultures suggested that a correlation, independent of buffer action, exists between the concentration of KH2PO4 initially present in the culture medium and the maintenance of a relatively high degree of antibacterial activity in the crude penicillin (8). The term "penicillin" is used here in its osriginal sense as defined by FLEMING (6) to ...

Eosinophils and their products are probably important in the pathophysiology of allergic diseases, such as bronchial asthma, and in host immunity to certain organisms. An association between environmental fungal exposure and asthma has been long recognized clinically. Although products of microorganisms (e.g., lipopolysaccharides) directly activate certain inflammatory cells (e.g., macrophages)...

Tillage practices on soils are effective for enhancing the soil’s structure and other physicochemical components. The present study was undertaken to investigate whether tilling of oil-contaminated can improve remediation efficiency soil. In study, activities principally carried out by just turning mixing in experimental bowls. Top soil obtained as pooled thoroughly mixed with spent engine oil ...

Contamination by petroleum products is a common occurrence in various depots Nigeria. Suleja depot recent times has recorded several spillages and fire incidence attributed to storage distribution. This study was aimed at determining the microbiological quality of soil water sources communities around Suleja, Soil samples were collected from defive control site. Microorganisms enumerated spread...

Antimicrobial activity of protease inhibitor isolated from Coccinia grandis (L.) Voigt. has been reported. A 14.3 kDa protease inhibitor (PI) was isolated and purified to homogeneity by ammonium sulfate precipitation (20-85% saturation), sephadex G-75, DEAE sepharose column and trypsin-sepharose affinity chromatography from the leaves of C. grandis. The purity was checked by reverse phase high ...

Tannin acyl hydrolase produced extracellularly by the fungal strain Penicillium notatum NCIM 923 in mixed solid state fermentation of wheat bran and marigold flower in the ratio 4 : 1 was purified from the cell-free extract broth by ammonium sulphate fractionation followed by diethylaminoethyl-cellulose column chromatography. Tannase was purified by 19.89-fold with yield of 11.77%. The specific...

Rosa damascena petals were extracted by water, hexane and ethanol. The latter was further fractionated with chloroform, ethyl acetate and butanol. Rose oil and different petal extracts were evaluated against three fungi and eleven Gram-positive, Gram-negative and acid-fast bacteria. Rose oil and all extracts exerted broad spectrum antimicrobial activities against the tested organisms. The desce...