Human relaxin, a two-chain protein hormone, was synthesized by solid-phase peptide synthesis in combination with a novel thiol-protecting group strategy whereby the three disulfide bonds could be synthesized sequentially and without error. The final product was shown to be homogeneous by reversed-phase high performance liquid chromatography and electrophoresis and had the correct amino acid com...

The substantia nigra is among the richest pro-dynorphin terminal field regions in the rat brain. We therefore contrasted processing in this area to the known processing in the posterior pituitary. Fractionation of acid extracts of the posterior pituitary by gel filtration followed by analysis by radioimmunoassay indicated that the molar ratio of dynorphin A(l-17) to dynorphin A(l-8) averaged 1:...

We apply a recently developed method, multicanonical algorithm, to the problem of tertiary structure prediction of peptides and proteins. As a simple example to test the effectiveness of the algorithm, Met-enkephalin is studied and the ergodicity problem, or multiple-minima problem, is shown to be overcome by this algorithm. The lowest-energy conformation obtained agrees with that determined by...

PROTEIN HYDROLYSATE-INDUCED CHOLECYSTOKININ SECRETION FROM 1 ENTEROENDOCRINE CELLS IS INDIRECTLY MEDIATED BY THE INTESTINAL 2 OLIGOPEPTIDE TRANSPORTER PEPT1 3 4 5 6 Alice P. Liou, Diana I. Chavez, Elvis Espero, Shuzhen Hao, Stephen A. Wank, Helen E. 7 Raybould 8 9 10 11 12 13 Department of Anatomy, Physiology, and Cell Biology 14 School of Veterinary Medicine, University of CaliforniaDavis 15 D...

Mass spectrometry-based peptidomic approaches are powerful techniques to detect and identify the peptide content of biological samples. The present study invest igated the l imitat ions of pept idomic approaches using trimethylammonium butyrate isotopic tags to quantify relative peptide levels and Mascot searches to identify peptides. Data were combined from previous studies on human cell lines...

I N T R O D U C T I O N T h e m a m m a l i a n ant id iure t ic ho rmone , vasopressin, modif ies the p e r m e a b i l i t y to wa t e r of the distal n e p h r o n and t he r eby accelera tes osmotic equ i l ib ra t ion of the fo rming ur ine wi th the renal interst i t ial fluid (4). Similar ly , neu ro hypophysea l pept ides increase the ra te of wa t e r m o v e m e n t a long an osmot ic...

The human gastric tumoral cell line HGT-1 was previously shown to contain a membrane somatostatin receptor negatively coupled to adenylate cyclase through a pertussis toxin-sensitive inhibitory GTP-binding regulatory protein (Gi) (Reyl-Desmars, F., Laboisse, C., and Lewin, M. J. M. (1986) Regul. Pept. 16, 207-215). In this study, we have solubilized this receptor in a free unoccupied form using...

Urea denaturation of allozymes was used to provide finer resolution of allelic states within classes of different electrophoretic mobility. This method gives perfectly repeatable results. About 170 isogenic strains for the O chromosome of Drosophila subobscura, derived from two natural populations, were constructed. Their gene arrangements were studied, as well as eight polymorphic genes locate...

1 Dodick DW, Goadsby PJ, Spierings ELH, Scherer JC, Sweeney SP, Grayzel DS. Safety and effi cacy of LY2951742, a monoclonal antibody to calcitonin gene-related peptide, for the prevention of migraine: a phase 2, randomised, double-blind, placebo-controlled study Lancet Neurol 2014; published online Aug 11. http://dx.doi.org/10.1016/S1474-4422(14)70128-0. 2 Goadsby PJ, Edvinsson L, Ekman R. Rele...

Positron emission particle tracking measures the trajectory of a single radioactively labelled tracer by coincident detection emitted annihilation photons. The technique enables non-invasive study dense opaque flows, with acting as small neutrally buoyant flow-follower. University Cape Town has established PEPT facility at iThemba LABS, utilising particles produced through radiochemical methods...