A modular approach has been developed for enzymatic ligation of peptides/proteins with oligonucleotides through the design tag phosphoramidites as adaptors, paving way towards streamlined production peptide/protein-oligonucleotide conjugates.

Proteolysis of food proteins changes their conformation and often generates bioactive peptides, leading to improved nutritional value techno-functional properties. Protein isolates that originated from plants are generally a mixture several proteins. Enzymatic hydrolysis such mixtures has drawback, as it is unclear which in the hydrolysed, poorly reproducible outcomes. In present study, we appl...

Human placenta hydrolysates (HPH) have a pronounced hepatoprotective effect, the molecular mechanisms of which are not well understood. As result de novo mass spectrometric sequencing and bioinformatics analysis peptides, 27 peptides were found in Laennec HPP preparation, (1) support inositol phosphate-dependent signaling pathways hepatocytes, (2) activate target proteins RARA, AMPK, (3) inhibi...

Abstract Major histocompatibility (MHC) bound viral peptides are the immune signatures to initiate selective and cross-reactive T cell responses. Identifying antigenic targets of adaptive immunity SARS-CoV-2 is a key towards understanding pathogenesis Sars-CoV-2 rapidly evolving mutants. We isolated immunopeptidomes identify naturally processed presented MHC-II (HLA-DR, HLA-DP) MHC-I (HLA-ABC) ...

Bioactive peptides (BPs) are multifunctional proteins that have a variety of activities and strong pharmacological effects. Due to the food-derived BPs' extensive spectrum health benefits numerous bioactivities, including antioxidant, immunological, anti-ageing, anti-inflammatory activities, research on these compounds has not been interrupted throughout past few decades. To balance daily rise ...