Protease 2A (2Apro) of coxsackievirus B3 (CVB3) plays a major role in viral replication. In case of infection, viral proteins are being synthesized from viral mRNA using host biosynthesis machinery. 2Apro of virus, after being synthesized, exhibits two critical functions, cleavage of viral proteins and breaking eukaryotic initiation factor 4G. The enzyme plays an essential role in viral replica...

Các hạt silica (SiO2) được cố định protein là vật liệu đầy triển vọng trong các lĩnh vực y sinh hay cảm biến học. Những nghiên cứu trước cho thấy ribosome L2 của vi khuẩn Escherichia coli liên kết mạnh với silica. Ngoài ra, A thụ thể bề mặt có tính ổn cao, nguồn gốc từ Staphylococcus aureus, sự giữa và kháng xem một những tương tác protein-protein kinh điển nhiều nhất hiện nay. Nghiên này tiến ...

automatic analysis of human facial expressions is one of the challenging problems in machine vision systems. it has many applications in human-computer interactions such as, social signal processing, social robots, deceit detection, interactive video and behavior monitoring. in this paper, we develop a new method for automatic facial expression recognition based on facial muscle anatomy and hum...

with advent and development of dna recombinant technology and advantages of p. pastoris expression system, fusion (f) protein of pprv expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against ppr disease. in this study, f gene of pprv nigeria 75/1 strain (1637 bp) was amplified using rt-pcr and purified. it was then clon...

background and objectives: hbha and mtb32c have been isolated from culture supernatants of mycobacterium tu- berculosis (m. tuberculosis) and mycobacterium bovis (m. bovis) and their immunogenicity previously studies have been confirmed. in this study, capability of constructed vector containing two mycobacterial immunodaminant antigens (mt- b32c-hbha), in producing new chimeric protein under t...

conclusions: our data showed that the recombinant mature lysostaphin protein produced by pet32a vector in e. coli system was very efficient. results: pcr and sequencing results confirmed the successful cloning of the target gene into the vector. the expression of protein was induced by iptg and high concentration of the recombinant protein was obtained via the purification process by affinity-c...

objective: induced pluripotent stem cells are generated from somatic cells by direct reprogramming. these reprogrammed pluripotent cells have different applications in biomedical fields such as regenerative medicine. although viral vectors are widely used for efficient reprogramming, they have limited applications in the clinic due to the risk for immunogenicity and insertional mutagenesis. acc...

Endoglucanase is an important enzyme participating in the hydrolysis of cellulose into oligosaccharides or glucoses can be used a variety industrial fields. A gene (code GL0183420 designated as eg9) 1398 bp encoding for endoglucanase family GH8 was identified from metagenomic DNA data bacteria humus surrounding wood hydrolyzed by white rot fungi. The 126 terminal nucleotides at 5' encode signal...

for high-throughput production of recombinant protein in escherichia coli ( e. coli ), besides important parameters such as efficient vector with strong promoter and compatible host, other important issues including codon usage, rare codons, and gc content specially at n-terminal region should be considered. in the current study, the effect of decreasing the percentage of gc nucleotides and opt...