نتایج جستجو برای: pfge profile
تعداد نتایج: 223267 فیلتر نتایج به سال:
BACKGROUND Drug resistance of Salmonella enterica serovar Typhi (Salmonella Typhi) to first-line antibiotics is emerging in Central Africa. Although increased use of fluoroquinolones is associated with spread of resistance, Salmonella Typhi with decreased ciprofloxacin susceptibility (DCS) has rarely been reported in Central Africa. METHODOLOGY/PRINCIPAL FINDINGS As part of a microbiological ...
To study comprehensive toxin profiles and the chromosomal diversity of current Japanese hospital-associated meticillin-resistant Staphylococcus aureus (HA-MRSA) strains, we conducted PCR-based identification of 28 toxin genes, and staphylococcal cassette chromosome mec (SCCmec) typing and PFGE analysis of 208 MRSA strains isolated from 100 hospitals throughout Japan. Of the tested HA-MRSA strai...
considering the circulation of bordetella pertussis clinical strains among populations with high vaccination coverage, it is necessary to have a proper understanding of this bacterium causing whooping cough. various techniques, which are available for studying b. pertussis, can facilitate a proper comparison between different populations. we genotypically analyzed a collection of two vaccine st...
Staphylococcus aureus causes mastitis in dairy cows, lambs, goats, and skin disorders pigs other animals. S. localized purulent infections that affect soft tissues, bones, organs humans. Using restriction patterns, the researchers want to isolate identify methicillin-resistant (MRSA) strains from cattle They also hope assess their genetic relatedness by comparing mecA1 mecA2 gene sequence discr...
Listeria monocytogenes is a foodborne human pathogen that causes systemic infection, fetal-placental infection in pregnant women causing abortion and stillbirth meningoencephalitis elderly immunocompromised individuals. This study aimed to analyse L. from different sources New Zealand (NZ) compare them with international strains. We used pulsed-field gel electrophoresis (PFGE), multilocus seque...
Introduction:Acinetobacter baumannii is an opportunistic nosocomial pathogen associated with multiple infections. This pathogen usually colonizes (first stage of microbial infection) host tissues that are in contact with the external environment. As one of the sites of entry in human hosts is the gastrointestinal tract, the pathogen must be capable of tolerating bile salts. However, studies ana...
Listeria monocytogenes strains that were isolated from 314 human listeriosis cases in Finland during an 11-year period were analyzed by O:H serotyping and pulsed-field gel electrophoresis (PFGE). Serotyping divided the isolates into five serotypes, the most common being 1/2a (53%) and 4b (27%). During the study period, the number of cases caused by serotype 1/2a increased from 22% in 1990 to 67...
BACKGROUND Campylobacter jejuni is the major cause of human bacterial gastroenteritis worldwide, and in a minority of cases, post-infectious complications may occur. ST-22 complex (usually Penner serotype 19) strains have been overrepresented among patients with postinfectious complications of campylobacteriosis. We here present a characterization of a collection of 27 Finnish C. jejuni strains...
BACKGROUND Multiple-locus variable-number tandem-repeat fingerprinting (MLVF) is based on multiplex PCR, utilizing variable number tandem repeat. Our goal was to compare the performance of MLVF in distinguishing clinical Staphylococcus aureus isolates with that of pulsed-field gel electrophoresis (PFGE), which has traditionally been the gold standard. METHODS Sixty-three clinically significan...
During 2015-2016, we evaluated the performance of whole-genome sequencing (WGS) as a routine typing tool. Its added value for microbiological and epidemiologic surveillance of listeriosis was compared with that for pulsed-field gel electrophoresis (PFGE), the current standard method. A total of 2,743 Listeria monocytogenes isolates collected as part of routine surveillance were characterized in...
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