The Korean native chickens (Woorimotdak™, KNC) and commercial broilers (Ross, CB) show obvious differences in meat flavor after cooking. To understand the contribution of protein and peptide for meat flavor, 2-dimensional (2-D) gel electrophoresis and matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry was performed. A total of 16 protein spots were differen...

Single-strand conformation polymorphism (SSCP) analysis is a simple and quick method for mutation detection. It depends on the separation of single-stranded (ss)DNA fragments in non-denaturing polyacrylamide gels. On occasion, the ssDNA bands can be very diffuse or appear against a heavily smeared background. Here, we demonstrate that the addition of denaturants, urea or formamide, to the gels ...

The promoter/operator/leader region of the trp operon of Shigell dysenteriae 16 has single base pair differences from the corresponding region of Escherichia coli at positions -24 and -13. The difference at -13 was shown to be responsible for the 90% reduction in promoter function characteristic of the trp operon of S. dysenteriae. The base pair difference at position -13 also renders the opera...

Objective: Classical Hodgkin lymphoma (HL) consists of neoplastic Hodgkin and Reed-Sternberg cells (HRSC) and a nonneoplastic micromilieu that greatly outnumbers the HRSC. Studies on HRSC-related prognostic biomarkers have been unsuccessful, but the microenvironmental composition is of prognostic importance. Recently, the number of CD68-positive macrophages was correlated with adverse survival ...

Over the past decade, 3D culture models of human and animal cells have found their way into tissue differentiation, drug development, personalized medicine tumour behaviour studies. Embryoid bodies (EBs) are in vitro cultures established from murine pluripotential stem cells, whereas tumoroids patient-derived cultures. This thesis aims to describe a new implication an embryoid body model charac...

HIV entry into CD4+ cells requires interaction with a cellular receptor, generally either CCR5 or CXCR4. We have previously reported the case of an HIV-infected patient in whom viral replication remained absent despite discontinuation of antiretroviral therapy after transplantation with CCR5{Delta}32/{Delta}32 stem cells. However, it was expected that the long-lived viral reservoir would lead t...

Chloroplasts in differentiated bundle sheath (BS) and mesophyll (M) cells of maize leaves are specialized to accommodate C4 photosynthesis. This study provides a reconstruction of how metabolic pathways, protein expression and homeostasis functions, are quantitatively distributed across BS and M chloroplasts. This yielded new insights in cellular specialization. The experimental analysis was ba...