It is well known that the osmotic pressure of protein solutions deviates from ideal behavior at high concentrations. Our lab previously developed a free-solvent model for osmotic pressure that provides excellent prediction of the osmotic pressure for protein solutions at moderate ionic strength using only physically-realizable parameters. The model assumes that the hydrated proteins behave idea...

The common pond snail Lymnaea stagnalis sacrifices 40-60% of its extracellular fluid (ECF) including solutes to the surrounding environment to fully retract into its shell. Our objectives were to determine recovery time following such ECF loss and characterize mechanisms involved in recovering Na(+), the primary cationic osmolyte in this snail. Pallial fluid was initially collected post-stimula...

Stress (biotic or abiotic) is an unfavourable condition for an organism including fungus. To overcome stress, organism expresses heat-shock proteins (Hsps) or chaperons to perform biological function. Hsps are involved in various routine biological processes such as transcription, translation and posttranslational modifications, protein folding, and aggregation and disaggregation of proteins. T...

The selection of yeast strains adapted to fermentation stresses in their winegrowing area is a key factor produce quality wines. Twelve non-Saccharomyces native from Denomination Origin (D.O.) “Vinos de Madrid” (Spain), warm climate region, were tested under osmotic pressure, ethanol, and acidic pH stresses. In addition, mixed combinations between Saccharomyces cerevisiae strain practised. Phen...

BACKGROUND AND AIMS Optimization of osmotic dehydration in different plant cells has been investigated through the variation of parameters such as the nature of the sugar used, the concentration of osmotic solutions and the processing time. In micro-organisms such as the yeast, Saccharomyces cerevisiae, the exposure of a cell to a slow increase in osmotic pressure preserves cell viability after...