In this study, solid-phase extraction (SPE) is described using a novel fluorinated [heptadecafluorotetrahydrodecyl (C(10)H(4)F(17))] phase to isolate THC and its primary metabolite carboxy-THC from whole blood samples. SPE was performed in hydrophobic mode after samples of whole blood were precipitated with acetonitrile. After applying the sample to the SPE column in aqueous phosphate buffer (p...

Abbreviations: AIBN, 2,20-azo-bis-isobutyronitrile; ASE, Accelerated solvent extraction; CAR, Carboxen; DAD, Diode-array detector; DI-SPME, Direct-immersion solid-phase extraction; DSPE, Dispersive solid-phase extraction; EGDMA, Ethylene glycol dimethacrylate; ESE, Enhanced solvent extraction; ESI, Electrospray ionization; FD, Fluorescence detection; FLD, Fluorimetric detection; GC, Gas chromat...

Levetiracetam (LEV), an antiepileptic drug (AED) with favorable pharmacokinetic profile, is increasingly being used in clinical practice, although information on its metabolism and disposition are still being generated. Therefore a simple, robust and fast liquid-liquid extraction (LLE) followed by highperformance liquid chromatography method is described that could be used for both pharmacokine...

In this work, we report a method to quantify methylparaben, ethylparaben, propylparaben and butylparaben in urban waters of São Carlos (São Paulo State, Brazil) by online solid phase extraction-liquid chromatography tandem mass spectrometry (SPE-LC-MS/MS) system. The achieved ng L-1 limits with good accuracy precision (internal standards for each paraben were used). total run cycle time takes 9...

BACKGROUND Busulfan (Bu) requires therapeutic drug monitoring (TDM) in subjects undergoing a conditioning regimen for hematopoietic stem cell transplantation (HSCT). To speed up the procedure and increase reproducibility, we improved our routine LC-MS/MS assay using the on-line solid-phase extraction (SPE) of samples. METHODS A protein precipitation (PP) step was performed before the on-line ...

Bioanalysis is sub-discipline of analytical chemistry covering the quantitative measurement of drugs and their metabolites in biological systems. Whereas Liquid chromatography mass spectrometry is a technique which is widely used for the quantification of drug from biological fluid. This article reviews the most recent advances in sample preparation, separation analysis and different type of ca...

Extraction of trace amounts of toxic heavy metals from complicated environmental matrixes is a  challenging task. The aim of this investigation is the comparison of the extraction efficiencies and other related experimental variables ( chemicals, effect of pH, potential interfering ions) on the determination of inorganic lead ( ) which is present in waste water of dye industries. Compared techn...

This article presents a comparative study of selected deproteinization-, liquid–liquid-extraction- (LLE), and solid-phase-extraction (SPE)-based procedures for the isolation doxorubicin (DOX) daunorubicin (DAU) as an internal standard (IS) from rat tissue samples. During experiments, all samples were analyzed via liquid chromatography coupled with fluorescence detection (LC-FL), analytes being ...

extraction of trace amounts of toxic heavy metals from complicated environmental matrixes is a  challenging task. the aim of this investigation is the comparison of the extraction efficiencies and other related experimental variables ( chemicals, effect of ph, potential interfering ions) on the determination of inorganic lead ( ) which is present in waste water of dye industries. compared techn...

A molecularly imprinted polymer for recognising selectively 2,3,7,8tetrachlorodibenzodioxin (TCDD) was made by a new non-covalent method employing a “dummy” template. The proposed way represents a simplification of a synthetic scheme proposed by Lübke et al.[1] for covalent imprinting. Comparison of extraction yields of the novel polymer, a non imprinted polymer and an imprinting polymer, prepa...