Due to the proteomics revolution, multi-dimensional separation and detection instruments are required to evaluate many peptides and proteins in single samples. In this study, electrospray ionization (ESI) ion mobility spectrometry (IMS) was evaluated as an additional separation after HPLC separations. Common HPLC mobile phase compositions (solvents, acid modifiers, and buffers) were assessed fo...

A rapid method for the isolation and high-performance liquid chromatographic (HPLC) determination of sulfamethazine (SMZ) in pork tissues (kidney, liver, and muscle) without using organic solvents is developed. The isolation is performed by homogenization with an acid solution using an ultrasonic-homogenizer, followed by centrifugation. The HPLC analyses are performed using a reversed-phase C(4...

The absorbed ester is hydrolyzed in the intestinal mucosa and in portal circulation [3]. Estimation of cefuroxime Axetil has been described by spectrophotometry [3-5], HPTLC [6-8] and HPLC [918]. HPLC methods are available in United State Pharmacopoeia [19] and in British pharmacopoeia [1]. The column in the official methods is Trimethylsilane bonded-phase. The mobile phase consisting of 0.2M a...

zataria multiflora boiss is used in traditional folk remedies as antiseptic, analgesic, carminative, anthelmintic medication. the main components of zataria multiflora are phenolic compounds such as thymol and carvacrol. the aim of this study was developed a simple and rapid method for determination of the thymol in different extracts of zataria multiflora by hplc method. the dried aerial parts...

A simple and reliable HPLC method for determination of rat plasma levels of clinically used acetylcholinesterase (AChE) reactivators (HI-6 and obidoxime) is presented in our study. Separation was carried out by HPLC using an octadecyl silica stationary phase and a mobile phase consisting of 24% acetonitrile and containing 5 mM sodium octanesulfonate and 5 mM tetramethylammonium chloride (pH 2.3...

A convenient and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for angiotensin I-converting enzyme inhibitory peptides assay was described in this paper. The mobile phase consisted of 70% A (0.05% TFA and 0.05% triethylamine in water, pH = 2.9-3.3) and 30% B (100% acetonitrile) using an Isogradient program. The flow rate was 0.5 mL/min. The absorb wavelength wa...

Monomeric and oligomeric procyanidins present in cocoa liquors and chocolates were separated and quantified in four different laboratories using a normal-phase high-performance liquid chromatography (HPLC) method with fluorescence detection. Procyanidin standards through decamers were obtained by extraction from cocoa beans, enrichment by Sephadex LH-20 gel permeation chromatography, and final ...

MARCHEV, A., I. IVANOV, R. VRANCHEVA and A. PAVLOV, 2013. Solid phase extraction and HPLC determination of phloridzin in natural products. Bulg. J. Agric. Sci., Supplement 2, 19: 201–203 A reliable technique for routine analysis of phloridzin from natural products (apples and plums) combining solid phase extraction with Amberlite XAD4 and subsequent HPLC analysis was developed. The proposed met...

The retention behavior of phosphatidic acids (PA) and phosphatidic acid methyl esters (PM) was studied by reversed-phase ion-pair high-performance liquid chromatography (HPLC). The HPLC systems consisted of mobile phases of acetonitrile-methanol-water containing tetraalkylammonium phosphates (TAAP) and stationary phases of alkyl-bonded silica and polystyrene--<:1iviny1benzene resins. The lipid ...

Depending on their rearing density, female desert locusts Schistocerca gregaria epigenetically endow their offspring with differing phenotypes. To identify the chemical basis for such maternal transmission, we compared solitarious and gregarious locust egg pod foam using high performance liquid chromatography (HPLC). We revealed causal relationships between foam chemistry and hatchling phenotyp...