Traditional flavor analysis relies on gas chromatography coupled to mass spectrometry (GC-MS) methods. Here we describe an indirect method coupling volatile compound formation to an ARO9-promoter-LacZ reporter gene. The resulting β-galactosidase activity correlated well with headspace solid phase micro extraction (HS/SPME) GC-MS data, particularly with respect to the formation of rose flavor. T...

Objective(s):The present study reports the development and validation of a sensitive and rapid extraction method beside high performance liquid chromatographic method for the determination of crocetin in human serum. Materials and Methods:The HPLC method was carried out by using a C18 reversed-phase column and a mobile phase composed of methanol/water/acetic acid (85:14.5:0.5 v/v/v) at the flow...

The objective of this paper is to control the phase shifting by applying a bias DC voltage and changing the mechanical characteristics in electrostatically-actuated micro-beams. This problem can be more useful in the design of micro-phase shifters, which has not generally been investigated their mechanical behavior. By presenting a mathematical modeling, Galerkin-based step by step linearizatio...

BACKGROUND RNA from sorted cell populations is crucial in many instances. We therefore compared four current protocols for RNA isolation, with regard to mRNA yield and purity. Moreover, we examined the effects on RNA recovery caused by different storage reagents. METHODS Small populations of K562 cells or PMBC were sorted into the lysing reagent and subjected to RNA extraction, employing eith...

In this study, hollow fiber liquid phase microextraction (HF-LPME) coupled with highperformanceliquid chromatography (HPLC) with UV detection was applied for preconcentrationand determination of Diclofenac sodium in biological fluids. Parameters affecting the extractionprocess including pH of donor phase and acceptor phase, type of extraction solvent, stirring rate,extraction time, and salt add...

Two-phase flow regimes are affected by conduit position, alignment, geometry, flow direction, physical characteristics, and flow rate of each phase as well as the heat flux toward the boundaries. Due to the importance of two-phase flow, numerous regimes have been identified. The first step in studying micro-bubble formation inside a venturi tube is to recognize the type of flow regimes. In this...