This procedure is the method of choice for purification of mammalian genomic DNA from monolayer cultures when large amounts of DNA are required, for example, for Southern blotting. Approximately 200 µg of mammalian DNA, 100-150 kb in length, is obtained from 5 × 107 cultured aneuploid cells (e.g., HeLa cells).

To investigate the regulation of drug absorption from the small intestine by the enteric nervous system (ENS), the vascular-luminal perfusion study and the in-vitro transport study were performed by employing phenol red as a poorly absorbable model compound. The effect of ENS on the intestinal absorption of phenol red was examined by adding epinephrine, an adrenergic agonist, or bethanechol, a ...

Bacillus stearothermophilus BR219, isolated from river sediment, degraded phenol at levels to 15 mM at a rate of 0.85 mumol/h (4 x 10(6) cells). The solubilized phenol hydroxylase was NADH dependent, exhibited a 55 degrees C temperature optimum for activity, and was not inhibited by 0.5 mM phenol.

Biodegradation of phenol is a major focus of toxic organic compound degradation by microorganisms isolated from polluted areas. An increasing number of bacteria and fungi possessing unique biodegradation capabilities have been isolated in recent years. In this study a new isolate, Rhodococcus erythropolis SKO-1, from polluted soils in the Tehran oil refinery region, is reported. Identificati...

Background: Phenol is an aromatic pollutant in industrial wastes that in combination with salts is highly toxic for all forms of life. Phenol elimination is the foremost challenge to meet the goal of pollutant-free environment. Objective: The present study was carried out to isolate phenol degrading bacteria which can degrade phenol under saline conditions and...