Phenylalanine ammonia-lyase, which irreversibly deaminates phenylalanine and tyrosine, markedly inhibited the growth of human leukemic and murine L5178Y lymphoblasts in vitro but had a negligible effect on resting (nondividing) normal lymphocytes. These studies also demonstrated that phenylalanine ammonia-lyase inhibited DNA synthesis in leukemic cells at least as effectively as did asparaginas...

Using DNA probes specific for the three members of the bean (Phaseolus vulgaris L.) phenylalanine ammonia-lyase (PAL) gene family, we have studied the effects of the product of the PAL reaction, trans-cinnamic acid (CA), on the appearance of individual PAL transcripts in suspension cultured bean cells. Concentrations of CA in excess of 10(-4) molar inhibited appearance of elicitor-induced trans...

Phenylalanine ammonia-lyase, which irreversibly deaminates phenylalanine and tyrosine, markedly inhibited the growth of human leukemic and murine L5178Y lymphoblasts in vitro but had a negligible effect on resting (nondividing) normal lymphocytes. These studies also demonstrated that phenylalanine ammonia-lyase inhibited DNA synthesis in leukemic cells at least as effectively as did asparaginas...

The aim of this study was to evaluate the physicochemical parameters mangoes for export. results revealed that lose firmness after three days storage and pulp turns yellow-orange. sugar content increases progressively along time. However, phenylalanine ammonia-lyase (PAL) activity decreases with time confirming decrease phenol content. As polyphenoloxidases (PPO) peroxidases (POD), it increased...

The first enzyme of the phenylpropanoid pathway, Phe ammonia-lyase (PAL), is encoded by four genes in Arabidopsis thaliana. Whereas PAL function is well established in various plants, an insight into the functional significance of individual gene family members is lacking. We show that in the absence of clear phenotypic alterations in the Arabidopsis pal1 and pal2 single mutants and with limite...

Cucumber (Cucumis sativa) leaves infiltrated with Pseudomonas syringae pv. syringae cells produced a mobile signal for systemic acquired resistance between 3 and 6 h after inoculation. The production of a mobile signal by inoculated leaves was followed by a transient increase in phenylalanine ammonia-lyase (PAL) activity in the petioles of inoculated leaves and in stems above inoculated leaves;...

Genome-wide identification and phylogenetic and syntenic comparison were performed for the genes responsible for phenylalanine ammonia lyase (PAL) and peroxidase A (POX A) enzymes in nine plant species representing very diverse groups like legumes (Glycine max and Medicago truncatula), fruits (Vitis vinifera), cereals (Sorghum bicolor, Zea mays, and Oryza sativa), trees (Populus trichocarpa), a...

Phenylalanine ammonia-lyase (PAL) gene is known to be expressed in plants, and is involved in the differentiation, growth and synthesis of secondary metabolites. However, its expression in fungi remains to be explored. To understand its expression in mushroom fungi, the PAL gene of the edible mushroom Flammulina velutipes (Fvpal) was cloned and characterized. The cloned Fvpal consists of 2,175 ...

In this study it was aimed to investigate inducibility of secondary metabolism in Astragalus chrysochlorus by yeast extract which is known to cause the synthesis of defensive phenolic metabolites. Cell suspension cultures of Astragalus chrysochlorus responded to elicitor treatment (10 gl(-1) yeast extract) by increasing phenylalanine ammonia lyase (PAL E.C. 4.3.1.5) activity the key enzyme of p...

Wounding during the preparation of fresh-cut lettuce induces the synthesis of phenylalanine ammonia lyase (PAL, EC 4.3.1.5), and the synthesis and accumulation of specific phenolic compounds (e.g., chlorogenic acid) that contribute to subsequent tissue browning. Increased PAL activity and phenolic content were not induced in non-wounded lettuce that was kept in close proximity to wounded tissue...