نتایج جستجو برای: phosphotungstic acid

تعداد نتایج: 747454  

2003

None of the methods which have been used for isolating Z-(+)lysine from plant extracts and protein hydrolysates is ideal. The oldest method utilizes phosphotungstic acid to precipitate the basic nitrogenous compounds which must be fractionated subsequently (1, 2). In the Kossel-Kutscher silver sulfate-barium hydroxide fractionation (3) the lysine remaining in the final filtrate must be precipit...

Journal: :Clinical chemistry 1965
J B O'SULLIVAN J O FRANCIS N KANTOR

‘J’HE MOST COMMONLY USED METHODS for the estimation of uric acid are those colorimetric procedures utilizing the reduction of phosphotungstic acid (1-7). Since these procedures are not specific for uric acid, an enzymatic procedure utilizing uricase, which oxidizes uric acid to allantoin, has been employed by a number of investigators (8-12). The enzymatic method, dependent upon the absorption ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2006
Jiri G Safar Holger Wille Michael D Geschwind Camille Deering Diane Latawiec Ana Serban David J King Giuseppe Legname Karl H Weisgraber Robert W Mahley Bruce L Miller Stephen J Dearmond Stanley B Prusiner

Prions are composed solely of an alternatively folded isoform of the prion protein (PrP), designated PrP(Sc). The polyoxometalate phosphotungstic acid has been used to separate PrP(Sc) from its precursor PrP(C) by selective precipitation; notably, native PrP(Sc) has not been solubilized by using nondenaturing detergents. Because of the similarities between PrP(Sc) and lipoproteins with respect ...

Journal: :Plant physiology 1983
M Uemura S Yoshida

An effective method for the isolation of plasma membrane from light-grown winter rye seedlings (Secale cereale L. cv Puma) was established using a liquid two-polymer phase separation. The conditions for the specific partition of plasma membrane into the polyethylene glycol-enriched upper phase were examined, including variations in the polymer concentration, buffer system, pH, and NaCl addition...

Journal: :Clinical chemistry 1975
J W Nelson K K Batra

We describe an automated colorimetric method for determination of uric acid in serum or urine by use of an AutoAnalyzer II or SMA 12/60 (Technicon Corp.). The method depends on reduction of a ferric-phenanthrolirle complex by uric acid under acidic conditions to a ferrous-phenanthroline complex, which absorbs at 505 nm. Advantages of this method over other methods now in use are that color and ...

Journal: :The Biochemical journal 2005
J C Drummond C Funk

The chemistry of rice-polishings has attracted many workers ever since it was shown that there occurred in the polishings a substance, apparently of a relatively simple chemical constitution, which had a powerful curative effect when administered in cases -of beri-beri, or experimental beri-beri, produced by a diet of polished rice. Practically all the communications which have appeared on the ...

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