It is well known that peripheral lymphocytes rarely divide in tissue culture; indeed, they have usually been considered to be the end cell in the pathway of differentiation. However, in the presence of certain mitogenic agents, of which phytohemagglutinin (PHA) is the best known example, a striking transformation occurs; the cells increase their over-all metabolic activity, enlarge, and divide....

Plasma lipoproteins with hydrated densities less than 1.063 g/ml, very low density, intermediate density, and low density lipoproteins, suppress mitogen-activated inductive biochemical events in lymphocytes. The ability of these lipoproteins to inhibit phytohemagglutinin (PHA)-enhanced incorporation of 32P into lymphocyte phospholipids correlates directly with the amount of associated apoprotei...

Subcellular fractions were isolated by differential centrifugation from pure suspensions of human blood lymphocytes incubated with and without phytohemagglutinin (PHA). Between 30 and 120 min after addition of PHA to intact cells, redistribution of acid hydrolases (beta glucuronidase, acid phosphatase), from a 20,000 g x 20 min granular fraction into the corresponding supernatant, was observed....

The insecticidal activity of plant lectins against a wide range of insect species have been intensively studied. Understanding the mechanism of the toxicity of lectins is one of the studied aspects. In the present research, the first step was determine the effect of phytohemagglutinin (PHA) on the development, fecundity and mortality of grain aphid. Next, the effect of PHA lectin on the activit...

Artemisinin derivatives are potent antimalarial compounds that may have immunomodulatory properties. Artesunate (range 0.01-2 mirog/ml) or dihydroartemisinin (range 0.01-8 microg/ml; DHART) were added to peripheral blood mononuclear cells (PBMC) or whole blood (WB) cultures before or simultaneously upon stimulation with phytohemagglutinin (PHA), a T cell mitogen. Lymphoproliferation was then me...

Agaricus blazei Murill is an edible and medicinal mushroom. In the previous study, we have proved that extracts of A. blazei inhibit human peripheral blood mononuclear cell (PBMC) proliferation activated with phytohemagglutinin (PHA). Currently, we purified 4-hydroxy-17-methylincisterol (4-HM; C21H33O3) from A. blazei investigated its regulatory effects on cytokine productions and cell prolifer...

A culture system has been developed that promotes growth of clonogenic lymphoma cells of some patients with intermediate and high-grade malignant lymphoma. The formation of colonies in bone marrow, lymph nodes, and peripheral blood samples is best supported by human plasma. Colony formation of some patients was dependent upon growth factors, which in this study were added in the form of medium ...

During long-term incubation of human peripheral blood lym phocytes with phytohemagglutinin (PHA), adenosine cyclic 3': 5'-monophosphate (cyclic AMP) phosphodiesterase increases about 5-fold and guanosine cyclic 3':S'-monophosphate (cyclic GMP) phosphodiesterase increases about i 0-fold in specific activity. Under similar conditions, there is little change in either basal or fluoride-stimulated ...

People with diabetes mellitus have an increased risk for infections, however, there is still a critical gap in precise knowledge about altered immune mechanisms this disease. Since diabetic INS C94Y transgenic pigs exhibit elevated blood glucose and stable phenotype soon after birth, they provide favorable model to explore functional alterations of cells early stage vivo . Hence, we investigate...

We studied the effect of phytohemagglutinin on the surface charge density and 90° light scattering of pea thylakoids as a function of pH (from pH 6.0 to pH 7.8). These pH-dependent modifications were interpreted in terms of the membrane conformational changes. Lectin induced a decrease in LS level due to the increase in aggregation of the particles at all pH studied. These changes were accompan...