The pharmacological effects of 4-phenyl-2-trichloromethyl-3H-1, 5-benzodiazepine hydrogen sulfate (PTMB), a novel synthetic benzodiazepine, were examined in mice. In the elevated plus-maze test of anxiety, 0.3-1 mg/kg diazepam ip (F(3,53) = 3.78; P<0.05) and 1-10 mg/kg PTMB ip increased (F(5,98) = 3.26; P<0.01), whereas 2 mg/kg picrotoxin ip decreased (F(3,59) = 8.32; P<0.001) the proportion of...

This study investigated the role of the dorsomedial prefrontal cortex (dmPFC) on the activity of the hypothalamus-pituitary-adrenal axis and memory consolidation in young and aged rats. The messenger RNA (mRNA) expression of several gamma-aminobutyric acid (GABA) and glutamate receptor subunits were also evaluated in the prefrontal cortex (PFC) of young and aged rats. Microinjections of picroto...

C-fiber depletion results in expansion of low threshold somatosensory mechanoreceptive fields. In this study, we investigated the role of intact C-fibers in GABAA-mediated inhibition in barrel cortical neurons. We used electronically controlled mechanical stimulation of whiskers to quantitatively examine the responses of barrel cells to whisker displacements. After systemic injection of picroto...

33 Low-voltage fast-(LVF) and hypersynchronous-(HYP) seizure onset patterns can be recognized 34 in the EEG of epileptic animals and patients with temporal lobe epilepsy. Ripples (80-200 Hz) 35 and fast ripples (250-500 Hz) have been linked to each pattern, with ripples predominating 36 during LVF seizures and fast ripples predominating during HYP seizures in the rat pilocarpine 37 model. This ...

in traditional system of indian medicine, c.bonducella is widely used for its antipyretic, antiperiodic, anticonvulsive, and antiparalytic activities. for assessing anticonvulsant activity, pentylenetetrazole, maximal electro shock, strychnine- and picrotoxin-induced convulsions models were used. diazepam was used as a standard reference for all models except maximal electro shock model, wherei...

In traditional system of Indian medicine, C.bonducella is widely used for its antipyretic, antiperiodic, anticonvulsive, and antiparalytic activities. For assessing anticonvulsant activity, pentylenetetrazole, maximal electro shock, strychnine- and picrotoxin-induced convulsions models were used. Diazepam was used as a standard reference for all models except maximal electro shock model, wh...

This is an attempt to investigate the effect of gamma-aminobutyric acid (GABA), a well-known major inhibitory neurotransmitter in the central nervous system, on the blood pressure response in rats and to elucidate the mechanism of its action. GABA injected into a femoral vein of the rat produced a dose-related fall in blood pressure followed by a secondary pressor response. The depressor respon...

We have studied mechanisms underlying long-term potentiation (LTP) in the medial and lateral amygdala using in vitro slice preparations. In normal bathing medium, LTP was not induced by tetanic stimulation (100 pulses at 100 Hz). However, in the presence of a GABAA blocker, picrotoxin or bicuculline, LTP was reproducibly induced in both medial and lateral amygdala. In the medial amygdala, the L...

The anticonvulsant effects of 3,4-Dimethoxy toluene (DMT), the major constituent of the date palm (Phoenix dactylifera L.) spathe, were investigated using pentylenetetrazole (PTZ), picrotoxin (Pic), nicotine (Nic) and maximal electroshock (MES)-induced seizure models. In PTZ (85 mg/kg)-induced seizures, the intraperitoneally injection of DMT at a dose of 100 mg/kg significantly delayed the onse...