Cysteine proteases are an important class of enzymes implicated in both developmental and defense-related programmed cell death and other biological processes in plants. Because there are dozens of cysteine proteases that are posttranslationally regulated by processing, environmental conditions, and inhibitors, new methodologies are required to study these pivotal enzymes individually. Here, we...

Abstract The replacement of fishmeal (FM) with economical and nutritious FM alternatives including plant animal-based protein ingredients has become a global research priority. However, the presence several anti-nutritional factors (ANFs) in these may impair nutrient utilization fish. supplementation exogenous protease as feed additives could be an effective approach to improve digestibility al...

The objective of this study was focussed on phytochemical screening, in vitro antioxidant and antiprotease activities various solvent extracts Muehlenbeckia platyclada root. roots were washed thoroughly, shade dried coarsely powdered. powdered material successively extracted with hexane, chloroform methanol using soxhlet apparatus. Preliminary screening for carbohydrates, proteins, alkaloids, p...

Protease activities in allergen sources are thought to be involved in triggering allergic inflammation through the disruption of epithelial barrier or the induction of proinflammatory cytokines. Protease allergens may also work as type 2 helper T cell (TH2) adjuvants through the cleavage of cell surface receptors. Here, we report molecular cloning and immunochemical characterization of a new Ja...

Penicilliurn expamum grown in a medium with rice husk as a carbon source produced an extracellular protease. The protease enzyme was isolated from culture broth by fractionation with acetone and column chromatography on Sephadex G- 100 and DEAE A-50. The protease enzyme was purified about 17.47 fold, with a recovery of 14%. The purified protease was homogenous on SDS polyacrylarnide disc ge...

Polyprotein processing is a major strategy used by many plant and animal viruses to maximize the number of protein products obtainable from a single open reading frame. In Sesbania mosaic virus, open reading frame-2 codes for a polyprotein that is cleaved into different functional proteins in cis by the N-terminal serine protease domain. The soluble protease domain lacking 70-amino-acid residue...

conclusions in this study, we isolated b. licheniformis producing an organic solvent-stable protease from oil-contaminated soil. the protease was stable and active in various organic solvents. by purification, the protease could be used as a biocatalyst for organic solvent-based enzymatic synthesis. results according to 16srdna sequencing, strain isa9 was identified as a new strain of bacillus ...

Deg/HtrA proteases are a large group of ATP-independent serine endoproteases found in almost every organism. Their usual domain arrangement comprises a trypsin-type protease domain and one or more PDZ domains. All Deg/HtrA proteases form homo-oligomers with trimers as the basic unit, where the active protease domain mediates the interaction between individual monomers. Among the members of the ...

Protease inhibitors play a role in regulating proteases during cellular development and in plant defense. We cloned and sequenced cDNA encoding six protease inhibitors expressed in soybean roots infected with soybean cyst nematode (SCN) and determined their expression patterns. Four of these protease inhibitors are novel and have not been reported previously. Using RT-PCR, we measured the relat...

BACKGROUND Proteases play an important role in virulence of many human, plant and insect pathogens. The proteinaceous protease inhibitors of plant origin have been reported widely from many plant species. The inhibitors may potentially be used for multiple therapeutic applications in viral, bacterial, fungal diseases and physiological disorders. In traditional Indian medicine system, Cassia tor...