We developed sugarcane plants with improved resistance to the sugarcane borer, Diatraea saccharalis (F). An expression vector pGcry1Ac0229, harboring the cry1Ac gene and the selectable marker gene, bar, was constructed. This construct was introduced into the sugarcane cultivar FN15 by particle bombardment. Transformed plantlets were identified after selection with Phosphinothricin (PPT) and Bas...

Abstract The black orchid ( Coelogyne pandurata Lindley) is one of the native Indonesian species. In its natural habitat, existence threatened by continuous forest exploitation. Coveted in global market for hybridization purposes, still considered difficult to be grown cultivation and may take years flower. Its conservation breeding carried out through a combination tissue culture mutagen appli...

DNA methylation is believed to play important roles in regulating gene expression in plant growth and development. Paulownia witches' broom (PaWB) infection has been reported to be related to gene expression changes in paulownia plantlets. To determine whether DNA methylation is associated with gene expression changes in response to phytoplasma, we investigated variations in genomic DNA sequenc...

PENG, Z. S., C. K. LUO, P. CAI, Z. C. MAO, C. Y. KANG and J. YANG, 2007. Rapid propagation of the medicinal plant Pinellia ternata by in vitro leaves culture. Bulg. J. Agric. Sci., 12: 01-06 Pinellia ternata (Thunb) Breit is an important medicinal plants. Its tubers,being called as Banxia in Chinese words, were a crude drug of traditional Chinese medicine and some pharmaceutical products. For s...

A protocol has been devised for enhanced in vitro regeneration of critically endangered Mantisia spathulata Schult. and Mantisia wengeri Fischer. Highest Bud Forming Capacity (BFC) of 6.10 +/- 0.55 with an average of 19.93 +/- 3.19 roots was obtained for M. spathulata within 5-6 weeks in Murashige and Skoogs (MS) medium supplemented with a combination of 10.0 microM of N6-benzyladenine (BA) and...

A large number of micropropagated plantlets of banana, Musa acuminata var. Nanjanagudu Rasabale (NR), that were developed from axillary shoot bud explants over 10 years ago were screened for genetic variation, if any, using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers. Of the 4000 in vitro plantlets, 11 were used for screening that involved shoot cult...

Aloe plants can be grown autotrophically in a maintenance-free, sterile, ex vitro environment. Excel, 15-5-15 plus minors fertilizer formulation and Plant Preservative Medium (PPM) biocide was used as a very economical basal medium. In vitro micropropagated plantlets were regenerated in Excel media with 2 g /L sucrose and 2.2 μM 6-Benzylamino purine (BA) and used to initiate ex vitro cultures i...

Somatic embryos and rooted plantlets have been regenerated from light-initiated embryogenic callus derived from mature embryos of Picea abies stored seeds. Under a 16 hour photoperiod, mature zygotic embryos were cultured on a modified half-strength Murashige-Skoog medium without NH4NO3 and supplemented with 5 mM glutamine, 4.5 pM N6-benzyladenine (BA) and 10.7 PM naphthalene-acetic acid (NAA) ...

Shoot tips and nodal segment explants to Rauwolfia serpentina when cultured on MS medium containing varying concentration of Benzyl adenine purine (BAP) and Benzyl adenine purine in combination with Indole butyric acid (IBA) produced multiple shoots. Maximum multiple shoots (85.6%) were found in static MS medium supplemented with 5.0 mg/ 1 BAP and 0.5 mg / 1 IBA along with 2.5% sucrose and 0.85...

In order to improve plant recovery from early heart-shaped Phaseolus embryos, several refinements were made to the embryo rescue technique developed previously in our laboratory. Using P. polyanthus Greenm. and P. vulgaris L. genotypes, a 20% increase in the germination rate was obtained by using Phillips salts and 25 μg.l-1 ABA. This germination medium improved the development of the embryos a...