Results: The mean urea nitrogen was 11 mg/dL in serum, 11 mg/dL in peritoneal fluid, and 469 mg/dL in urine. The mean creatinine was .9 mg/dL in serum, 1.0 mg/dL in peritoneal fluid, and 141 mg/dL in urine. Urea nitrogen and creatinine values in peritoneal fluid and serum were essentially identical. Urine urea nitrogen and creatinine values were significantly greater than serum and peritoneal v...

BACKGROUND In hemodialysis patients, plasma levels of total homocysteine are influenced by nutritional status in patients with chronic kidney disease. To investigate the association between serum homocysteine (Hcy) level as a marker of nutritional status and WBC counts as a marker of inflammation, a cross-sectional study was conducted on patients with end-stage renal disease (ESRD), who were un...

Urea recycling, with urea originating from catabolism of amino acids and hepatic detoxification of ammonia, is particularly relevant for ruminant animals, in which microbial protein contributes substantially to the metabolizable protein supply. However, the quantitative contribution of urea recycling to protein anabolism in calves during the transition from preruminants (milk-fed calves) to rum...

The objectives of this study were to relate concentrations of plasma (PUN) and milk (MUN) urea nitrogen to pregnancy rate in dairy cows and compare various methods of analysis and preparation of milk for measuring MUN. In two experiments, blood or milk samples were collected on the day of AI from Holstein cows (n = 160 and n = 155, respectively). Three methods of MUN analysis were compared. Two...

Twenty-five Landrace • Yorkshire sows (second through seventh parity) were randomly assigned to five dietary treatments in an experiment designed to estimate the isoleucine requirement of the sow during the first 3 weeks of lactation. The basal diet (.20% isoleucine), composed of oat groats, corn sugar, wood cellulose, soybean oil, vitamins, minerals, indispensable amino acids and L-glutamic ac...

BACKGROUND Nitrogen absorption from the large intestine is considered of limited value for supporting body protein synthesis in animals and humans, but it may be of benefit when the dietary supply of nitrogen for synthesis of dispensable amino acids (DAAs) is deficient. OBJECTIVE A whole-body nitrogen balance study was conducted to evaluate the impact of nitrogen absorption from the large int...

The effects of urea and rumen-degradable protein (RDP) on microbial growth, digestibility, and fermentation were examined using dual-flow continuous culture. The experimental design was a 4 x 4 Latin square with a 2 x 2 factorial arrangement of treatments. Factors were urea infusion (0.4 g/L of artificial saliva) and RDP concentration, and the treatments were as follows: 1) low RDP (8% of dieta...

In the earlier papers in this series (3, 4) data have been presented which indicate tha t the isolated perfused liver from a ra t fed 8'-methyldimethylaminoazobenzene 1 has a decreased ability to oxidatively catabolize amino acids. Such livers, however, show an increased capacity for incorporation of C14-1abeled amino acid into both liver and plasma proteins. A biochemical block or impairment i...

A simple method is described for directly determining serum urea nitrogen, wtihout deproteinization. The urea in 20 Ml of serum or plasma is reacted with diacetylmonoxime in the presence of thiosemicarbazide and cadmium ion under acid conditions. The absorbance of the resulting rose-purple solution is measured at 540 nm. Results agreed excellently with those obtained by a reference method that ...